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DOI :
10.3791/56631-v
January 26th, 2018
Chapters
0:05
Title
0:49
Isolation of Cerebellar Granular Neuron Progenitors (CGNP)
3:59
Cell Fixation and Chromatin Shearing
5:53
Preclearing
6:47
Preparation of Antibody-Coupled Beads
7:37
Chromatin Immunoprecipitation
8:51
Results: H3K79me2 ChIP-qPCR and ChIP-Seq Analysis
9:41
Conclusion
我们提出一种有效和可重复的方法, 分离和培养胚胎和产后脑组织中的神经祖细胞染色质沉淀 (芯片) 的组蛋白3赖氨酸 79 dimethylation (H3K79me2)-一个组蛋白标记位于组蛋白3的球状域。
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