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DOI :
10.3791/56662-v
•
9:51 min
December 13th, 2017
Chapters
0:05
Title
1:41
Isolation of Intestinal Organoids from Basement Matrix Domes in 24-well Plates
3:20
Fixation of Isolated Organoids
4:22
Blocking, and Primary and Secondary Antibody Incubation
6:41
Mounting Isolated Organoids
7:39
Results: Isolation and Immunolabeling of Intestinal Organoids
8:55
Conclusion
我们提出了从体内组织和体外基底基质 organoids 分离肠道3D 结构的协议, 并详细介绍了针对微管免疫标记优化的不同固定和染色方案,中心, 和连接蛋白以及细胞标记包括干细胞蛋白 Lgr5。
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