Name: transurethral instillation procedure in adult male mouse
Uploaded: 2017-11-02T15:00:00
Description: Watch this Scientific Journal Video about Transurethral Instillation Procedure in Adult Male Mouse at JoVE.com

作者没有任何致谢。

所有与动物有关的程序, 包括麻醉的方法和持续时间, 以及术后护理, 都与兽医进行了讨论, 并得到了机构动物护理和使用委员会 (IACUC) 的批准。附属机构。在这份手稿中, 所有的程序都是用8到15周大的 C57BL/6 雄性小鼠进行的, 该协议遵循 IACUC 在科罗拉多大学批准的动物保育指南. 
 1. 油管的制备 注: 为了灌输接种, 一根针连接到聚乙烯 (PE) 油管 (<strong class="x...

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本手稿详细介绍了经尿道灌注法在成年雄性小鼠中的应用。采用不同直径的 PE 管材, 可达到差异主播种面积。PE-50 是建议成功达到膀胱腔, 而较大的直径 PE-10 是用来达到 AP 和 SV (图 3A-b)。除了选择 PE 管的大小和靶器官的接种外, 其他因素, 如注入量的接种, 也发挥了作用的最终位置和分布的解决方案。我们的实验证明, 经尿道灌注100µL 的蓝色染料使用 PE-10 或 PE-50...

在以往的研究中, 采用经尿道灌注法作为建立膀胱疾病模型的方法之一1,2,3,4 , 可用于评估当地提供的治疗小鼠的效果。虽然动物模型不能完全重述人类的病症, 但在动物研究中的基本机制的识别更能更好地理解人类的查找障碍, 如间质性膀胱炎/膀胱疼痛综合症, 神经原膀胱炎, 自身免疫性膀胱炎, 前列腺炎症5。
在成年雄性小鼠身上进行的经尿道灌注手术比在成年雌性老鼠6中的膀胱灌注更具有技术挑战性。男性尿道的自然弯曲解剖和它的小直径使它在技术上难以完成经尿道插入导管。因此,详细说明对经尿道感染的小鼠泌尿道传染病的诱导6和查找源性炎症7,8,910,12,13,14,15通过膀胱插入导管之前只对雌性小鼠进行了概述。这份手稿的目的是提供一个 step-by 一步的描述的技术, 在男性小鼠经尿道注入的物质, 包括视频剪辑, 图像和插图。经尿道灌注手术的成年雄性小鼠可以执行的变量接种类似于以前所描述的雌性鼠标7和合并的其他技术, 如肌电图记录visceromotor 响应 (VMR)16。

<table>
	<tbody>
		<tr>
			<td>BD INTRAMEDIC Polyethylene Tubing/ PE 10</td>
			<td>BD Medical</td>
			<td>63019-004</td>
			<td></td>
		</tr>
		<tr>
			<td>BD INTRAMEDIC Polyethylene Tubing/ PE 50</td>
			<td>BD Medical</td>
			<td>63019-048</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Insyte-N Autoguard/ Angiocath</td>
			<td>BD Medical</td>
			<td>311411</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol (EtOH)</td>
			<td>Fisher Scientific</td>
			<td>BP2818500</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Needle Only 22 Gauge (G) 1 inch</td>
			<td>Becton Dickson</td>
			<td>205155</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Needle Only 27 Gauge (G) 1/2 inch</td>
			<td>Becton Dickson</td>
			<td>305129</td>
			<td></td>
		</tr>
		<tr>
			<td>Luer stub, 22 G X 0.5 in / Blunt Needle</td>
			<td>Instech</td>
			<td>LS22</td>
			<td></td>
		</tr>
		<tr>
			<td>Luer stub, 27 G X 0.5 in / Blunt Needle</td>
			<td>Instech</td>
			<td>LS27</td>
			<td></td>
		</tr>
		<tr>
			<td>6&#34; Long x 2&#34; Wide x 1&#34; Thick, Aluminum Oxide Sharpening Stone / Oil Stone</td>
			<td>Norton</td>
			<td>40204034</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical Lubricant Sterile Bacteriostatic Folilpac</td>
			<td>Surgilube</td>
			<td>281020543</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Fluriso</td>
			<td>13985-528-60</td>
			<td></td>
		</tr>
		<tr>
			<td>SomnoSuite Low-Flow Anesthesia System</td>
			<td>Kent Scientific</td>
			<td>SS-01</td>
			<td></td>
		</tr>
		<tr>
			<td>Dissecting Forceps, Curved</td>
			<td>vwr</td>
			<td>82027-392</td>
			<td></td>
		</tr>
		<tr>
			<td>BD 1 ml Syringe</td>
			<td>BD Medical</td>
			<td>309626</td>
			<td></td>
		</tr>
		<tr>
			<td>India Ink Solution., 0.2% in PBS buffer</td>
			<td>Alfa Aesar</td>
			<td>J61007</td>
			<td></td>
		</tr>
		<tr>
			<td>8~12 weeks old male C57B/6 Mice</td>
			<td>Jackson Lab</td>
			<td>#000664</td>
			<td></td>
		</tr>
	</tbody>
</table>

transurethral instillation procedure in adult male mouse

经尿道灌注可用于提供不同的解决方案与活性成分 (例如,药物, 化学药品, 细菌和病毒) 当地进入膀胱, 要么诱发动物模型膀胱病理或评估膀胱灌注治疗的有效性。大多数啮齿动物模型的下尿路 (查找) 病理是诱导雌性小鼠, 由于容易膀胱灌注的物质通过女性尿道。然而, 由于女性与男性之间的解剖差异, 经尿道灌注的雄性小鼠被认为是一个非常具有挑战性的过程, 并没有事先描述。在这份手稿中, 我们提供了一个详细的描述如何准备聚乙烯 (PE) 油管后插入到尿道的雄性鼠标。此外, 我们还讨论了理想的 PE 管的类型, 要根据所需的接种地点使用。此外, 我们还描述了如何准备一个成功的经尿道灌注的动物, 以避免对尿道的伤害, 并确保提供的解决方案的理想地点。手术的开始是通过缩回包皮和头来暴露尿道口的开口。其次, 用钝 non-crushing 钳抓住阴蒂, 稳定小弟弟和 PE 管。PE 导管首先插入到与动物身体平行的尿道道中, 然后通过倾斜导管来调整它的角度来操纵它以遵循尿道的自然曲率。这项技术可用于诱导小鼠膀胱病理模型和/或评估的有效性, 在雄性老鼠的治疗。

通过 pe 50 导管经尿道注入蓝染料后, 将染料滴入膀胱 (图 4A), 并通过 pe 10 导管在 SV 和 AP (图 4B) 着色中分别进行。为了评价经尿道电切术是否使接种的即刻回流到肾脏, 肾脏和输尿管被观察到, 然后注入100µL 的蓝色染料和荧光染料 (图 4A-b)。没有使用两种不同尺寸的 PE 导管和两种不同的染料的?...

Watch this Scientific Journal Video about Transurethral Instillation Procedure in Adult Male Mouse at JoVE.com

Transurethral Instillation Procedure in Adult Male Mouse

经尿道灌注是一项具有挑战性的手术, 在文献中没有得到很好的描述。本手稿的目的是描述一种技术, 经尿道插入导尿管膀胱灌注液体的活性物质进入膀胱和/或前列腺的成年雄性小鼠。

成年雄性小鼠经尿道灌注术

Transurethral instillation can be used to deliver different solutions with active ingredients (e.g., drugs, chemicals, bacteria, and viruses) locally into ...

Research

JoVE Journal

Immunology and Infection

Transurethral Induction of Mouse Urinary Tract Infection

经尿道诱导小鼠尿路感染

Uropathogenic bacterial strains of interest are grown on agar. Generally, uropathogenic E. coli (UPEC) and other strains can be grown overnight on ...

科研

免疫与感染

Retroviral Transduction of T-cell Receptors in Mouse T-cells

T细胞受体在小鼠T细胞的逆转录病毒转导

T-cell receptors (TCRs) play a central role in the immune system. TCRs on T-cell surfaces can specifically recognize peptide antigens presented by antigen ...

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

非手术治疗的小鼠气管内滴入肺和肺淋巴结流式细胞仪分析

Phagocytic cells such as alveolar macrophages and lung dendritic cells (LDCs) continuously sample antigens from the alveolar spaces in the 
lungs. LDCs, ...

Culturing Microglia from the Neonatal and Adult Central Nervous System

培养小胶质细胞的新生和成年的中枢神经系统

Microglia are the resident macrophage-like cells of the central nervous system (CNS) and, as such, have critically important roles in physiological and ...

Complete Thymectomy in Adult Rats with Non-invasive Endotracheal Intubation

完整的胸腺切除术的成年大鼠非侵入气管插管

Thymectomy in neonatal rodents is an established and reliable procedure for immunological studies. However, in adult rats, complications of hemorrhage and ...

Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

小动物模型尿路感染的实验研究: 男性和女性的经尿道插管

Urinary tract infections (UTI) are extremely common worldwide, incurring significant morbidity and healthcare-associated expenses. Small animal models, ...

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

成年斑马鱼模型中霍乱弧菌定植与腹泻的量化研究

Vibrio cholerae is best known as the infectious agent that causes the human disease cholera. Outside the human host, V. cholerae primarily exists in the ...

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

marinum 分枝杆菌感染成人斑马鱼结核的建模

Mycobacterium tuberculosis is currently the deadliest human pathogen causing 1.7 million deaths and 10.4 million infections every year. Exposure to this ...

A Model of Self-limited Acute Lung Injury by Unilateral Intra-bronchial Acid Instillation

单方支气管内酸灌输自我限制急性肺损伤模型

Selective intra-bronchial instillation of hydrochloric acid (HCl) to the murine left mainstem bronchus causes acute tissue injury with histopathologic ...

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

经口喂养和显微注射对成年蚊子进行实验性病毒感染

Mosquito-borne viruses (MBVs), which are infectious pathogens to vertebrates, are spread by many mosquito species, posing a severe threat to public ...