Name: transurethral instillation procedure in adult male mouse
Uploaded: 2017-11-02T15:00:00
Description: Watch this Scientific Journal Video about Transurethral Instillation Procedure in Adult Male Mouse at JoVE.com

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alle procedurer med dyr, herunder metode og varighed af anæstesi og postoperativ pleje, blev diskuteret med en dyrlæge og godkendt af institutionelle dyrs pleje og brug udvalg (IACUC) på den tilknyttet institutionen. I dette manuskript, alle procedurer blev udført med 8 til 15 uger gamle C57BL/6 mandlige mus og protokollen følger dyrs pleje retningslinjer godkendt af IACUC på University of Colorado.
 1. forberedelse af slangen Bemærk: for at indgyde inokulat,...

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Dette manuskript beskriver i detaljer en metode af transuretral instillation i voksne mandlige mus. Differential primære seeding område kan nås ved hjælp af PE rør af forskellige diametre. PE-50 anbefales for med held at nå urinblæren lumen, mens en større diameter PE-10 anvendes til at nå AP og SV (figur 3A-B). Ud over valget af størrelsen på PE rør og målorgan for podning spille andre faktorer, såsom omfanget af indpodet inokulum, også en rolle i slutningen ...

Transuretral instillation tilgang har været anvendt i tidligere undersøgelser som en af metoderne til at oprette gnavere modeller af blæren patologier1,2,3,4 og kan bruges til at evaluere de effektiviteten af lokalt leverede behandlinger i mus. Selvom dyremodeller ikke fuldt sammenfatte menneskelige patologier, giver identifikation af de underliggende mekanismer i dyreforsøg en bedre forståelse af de menneskelige LUT lidelser såsom interstitiel cystitis/blæresmerter syndrom, neurogen blærebetændelse, autoimmune blærebetændelse og prostata inflammation5.
Transuretral instillation procedure udføres på en voksen mand mus er mere teknisk udfordrende end intravesikal instillation i en voksen kvindelig musen6. Den naturligt buede anatomi af de mandlige urinrøret sammen med sin lille diameter gør det teknisk vanskeligt at gøre transuretral indsættelse af et kateter. Derfor detaljerede instruktioner for transuretral induktion af musen urinary spore infektion6 og LUT betændelse7,8,9,10,11 , 12 , 13 , 14 , 15 via blære-indsat kateter var tidligere skitseret for kvindelige mus kun. Dette manuskript har til formål at give en trinvis beskrivelse af teknikken for transuretral instillation af stoffer i mandlige mus herunder videoklip, billeder og illustrationer. Transuretral instillation procedurer i en voksen mand mus kan udføres med variabel inokulerede samme som tidligere beskrevet i den kvindelige mus7 og fusioneret med supplerende teknikker såsom electromyogram optagelser af visceromotor svar (VMR)16.

<table>
	<tbody>
		<tr>
			<td>BD INTRAMEDIC Polyethylene Tubing/ PE 10</td>
			<td>BD Medical</td>
			<td>63019-004</td>
			<td></td>
		</tr>
		<tr>
			<td>BD INTRAMEDIC Polyethylene Tubing/ PE 50</td>
			<td>BD Medical</td>
			<td>63019-048</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Insyte-N Autoguard/ Angiocath</td>
			<td>BD Medical</td>
			<td>311411</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol (EtOH)</td>
			<td>Fisher Scientific</td>
			<td>BP2818500</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Needle Only 22 Gauge (G) 1 inch</td>
			<td>Becton Dickson</td>
			<td>205155</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Needle Only 27 Gauge (G) 1/2 inch</td>
			<td>Becton Dickson</td>
			<td>305129</td>
			<td></td>
		</tr>
		<tr>
			<td>Luer stub, 22 G X 0.5 in / Blunt Needle</td>
			<td>Instech</td>
			<td>LS22</td>
			<td></td>
		</tr>
		<tr>
			<td>Luer stub, 27 G X 0.5 in / Blunt Needle</td>
			<td>Instech</td>
			<td>LS27</td>
			<td></td>
		</tr>
		<tr>
			<td>6&#34; Long x 2&#34; Wide x 1&#34; Thick, Aluminum Oxide Sharpening Stone / Oil Stone</td>
			<td>Norton</td>
			<td>40204034</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical Lubricant Sterile Bacteriostatic Folilpac</td>
			<td>Surgilube</td>
			<td>281020543</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Fluriso</td>
			<td>13985-528-60</td>
			<td></td>
		</tr>
		<tr>
			<td>SomnoSuite Low-Flow Anesthesia System</td>
			<td>Kent Scientific</td>
			<td>SS-01</td>
			<td></td>
		</tr>
		<tr>
			<td>Dissecting Forceps, Curved</td>
			<td>vwr</td>
			<td>82027-392</td>
			<td></td>
		</tr>
		<tr>
			<td>BD 1 ml Syringe</td>
			<td>BD Medical</td>
			<td>309626</td>
			<td></td>
		</tr>
		<tr>
			<td>India Ink Solution., 0.2% in PBS buffer</td>
			<td>Alfa Aesar</td>
			<td>J61007</td>
			<td></td>
		</tr>
		<tr>
			<td>8~12 weeks old male C57B/6 Mice</td>
			<td>Jackson Lab</td>
			<td>#000664</td>
			<td></td>
		</tr>
	</tbody>
</table>

transurethral instillation procedure in adult male mouse

Transuretral instillation kan bruges til at levere forskellige løsninger med aktive ingredienser (f.eks. narkotika, kemikalier, bakterier og vira) lokalt i urinblæren til enten fremkalde dyremodeller for blære patologier eller vurdere den effektiviteten af intravesikal behandlinger. De fleste gnavere modeller af nedre urinveje (LUT) patologier er induceret i hunmus grund at lette af intravesikal instillation af stoffer via den kvindelige urinrøret. Men på grund af anatomiske forskelle mellem kvindelige og mandlige LUT, transuretral instillation i en mandlig mus har været anset for en meget udfordrende procedure, og det er ikke tidligere beskrevet. I dette manuskript giver vi en detaljeret beskrivelse af hvordan man forbereder polyethylen (PE) rør til efterfølgende indsættelse i urinrøret mandlige musen. Derudover diskuterer vi de ideelle typer af PE rør anvendes afhængigt af det ønskede websted af podning. Desuden, vi beskriver punkt af punkt Sådan Forbered en animalsk nemlig en vellykket transuretral instillation til at undgå skade på urinrøret og sikre levering af løsningen til den ønskede placering. Proceduren, der er startet af tilbagetrækningskraften forhud og glans at udsætte indledningen af den urethral meatus. Næste, glans er forstået af stump ikke-knusning pincet til at stabilisere penis og PE rør. PE rør indsættes først i urethral meatus parallelt med det animalske organ, så dets vinkel er justeret ved at vippe kateter for at manøvrere det for at følge den naturlige krumning af urinrøret. Denne teknik kan bruges til inducerede murine modeller af blæren patologier og/eller vurdere effektiviteten af intravesikal behandlinger i mandlige mus.

Transuretral instillation af blå farvestof via PE 50 slanger resulterede i instillation af farvestof i urinblæren (figur 4A), og via PE 10 slanger i farvelægningsmetode SV og AP (figur 4B), henholdsvis. For at vurdere, om de udførte transuretral instillation forårsager en øjeblikkelig refluks af inokulum til nyrerne eller ej, observeret nyre og ureter efterfulgt af instillation af 100 µL af det blå farvestof og fluorescer...

Watch this Scientific Journal Video about Transurethral Instillation Procedure in Adult Male Mouse at JoVE.com

Transurethral Instillation Procedure in Adult Male Mouse

Transuretral instillation er en udfordrende procedure og er ikke blevet godt beskrevet i litteraturen. Formålet med dette manuskript er at beskrive en teknik til transuretral indsættelse af et kateter til intravesikal levering af væsker med aktive stoffer ind i urinblæren og/eller prostata hos voksne mandlige mus.

Transuretral Instillation Procedure i voksne mandlige mus

Transurethral instillation can be used to deliver different solutions with active ingredients (e.g., drugs, chemicals, bacteria, and viruses) locally into ...

Research

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