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DOI :
10.3791/56675-v
November 17th, 2017
Chapters
0:05
Title
1:05
Preparation of Pulled Pipettes
2:29
In Utero Electroporation
6:54
Brain Removal for Histological Preparation
8:06
Results: Expression of GFP After Electroporation
9:18
Conclusion
在稀疏的细胞群中, 可以通过诱导功能的丧失或增益来研究脑内基因的细胞自主功能。在这里, 我们描述在子宫内电穿孔, 以提供重组到稀疏的人群发育皮层神经元与 floxed 基因, 导致功能丧失在体内。
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