Name: in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur
Uploaded: 2017-11-14T15:00:00
Description: Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

我们感谢 Sevil Essig, 孙燕姿 Schroth, Verena 费舍尔, 卡嘉 Prystaz, 伊冯 Hägele 和安妮 Subgang 的优秀技术支持。我们还感谢德国研究基金会 (CRC1149, INST40/499-1) 和 AO 创伤基金会德国资助这项研究。

所有动物实验都遵守了对实验室动物的照顾和使用的国际规定, 并得到了区域管理当局的批准 (No. 1250、Regierungspr 和 #228; sidium T & #252; 宾, 德国)。所有小鼠每笼保持两到五只动物, 每个笼子上的 14 h 光, 10 小时黑暗的昼夜节律与水和食物提供了 ad 随意 . 
 1. 小鼠的外科材料和前处理的准备 <ol> <li> 对所有的外科材料进行消毒。使用灭菌温度为120-135 和 #176; C 用于20-30 分?...

<ol>
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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Age+and+ovariectomy+impair+both+the+normalization+of+mechanical+properties+and+the+accretion+of+mineral+by+the+fracture+callus+in+rats.">Age and ovariectomy impair both the normalization of mechanical properties and the accretion of mineral by the fracture callus in rats.</a> J Orthop Res. 19 (3), 428-435 (2001).</li><li>Nikolaou, V. S., Efstathopoulos, N., Kontakis, G., Kanakaris, N. K., Giannoudis, P. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+influence+of+osteoporosis+in+femoral+fracture+healing+time.">The influence of osteoporosis in femoral fracture healing time.</a> Injury. 40 (6), 663-668 (2009).</li><li>Haffner-Luntzer, M., Kovtun, A., Rapp, A. 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修改和故障排除:
本研究的主要目的是描述一个协议的使用 MRI 兼容外固定器股骨截骨在小鼠的能力, 以监测骨痂组织发展纵向软骨骨折愈合过程。custom-made 安装装置的设计, 用于插入外固定架, 确保了在重复扫描过程中的标准化位置。半自动组织分割允许分析的数量的纤维, 软骨, 骨组织的骨折骨痂。此外, 3D 重建的 MRI 图像允许可视化的软骨骨折愈合过程中每个单?...

二次骨折愈合是最常见的骨愈合形式。这是一个复杂的过程, 模仿 ontogenic 软骨骨化的具体方面1,2,3。早期骨折血肿主要由免疫细胞、肉芽组织和纤维组织组成。低氧张力和高生物力学菌株在骨折间隙中阻碍成骨细胞分化, 但促进前体细胞分化成软骨细胞4,5,6。这些细胞在损伤部位开始增殖, 形成软骨基质, 提供骨折骨的初始稳定性。在愈伤组织成熟过程中, 软骨细胞变得肥大、凋亡或分化成成骨细胞。cartilage-to-bone 过渡区的新生血管提供了高氧水平, 使骨组织的形成7。骨折间隙骨桥接后, 生物力学稳定性提高, 外骨折愈伤组织发生破重塑, 获得生理骨轮廓和结构3。因此, 骨痂中纤维、软骨和骨组织的数量提供了骨骼愈合过程的重要信息。扰或延迟愈合变得可看见通过改变愈伤组织的发展在人和小鼠8,9,10,11。可用的体内技术纵向监测骨痂组织发育的临床前骨折愈合研究使用小动物包括数字造影和µCT 成像12,13。然而, 这两种技术只能够区分矿化和 non-mineralized 组织。相比之下, MRI 提供了优秀的软组织对比, 因此可以识别软组织和软骨在骨折愈伤组织。
先前的工作显示, 在膜骨缺损愈合15中, 在小鼠关节骨折的14和活体mri 检查中, 有希望的结果。然而, 这两项研究也表明空间分辨率和组织对比度有限。我们先前证明了高分辨率的体内 MRI 对小鼠软骨骨折愈合过程中软痂形成的纵向评估的可行性16。在这里, 我们报告的协议, 使用 MRI 兼容外固定器股骨截骨在小鼠, 以监测骨痂组织发展纵向在软骨骨折愈合过程。custom-made 安装装置的设计, 用于插入外固定架, 确保了在重复扫描过程中的标准化位置。

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			<td class="xl65" height="21" style="width:254pt;height:15.75pt" width="338">Anaesthesia tube</td>
			<td class="xl65" style="width:295pt" width="393">FMI, Seeheim, Germany</td>
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			<td class="xl65" height="21" style="height:15.75pt">Artery forceps&#160;</td>
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			<td class="xl65">Systec, Wettenberg, Germany</td>
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			<td class="xl65">Stericlin, Feuchtwangen, Germany</td>
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			<td class="xl65" height="21" style="height:15.75pt">Avizo software</td>
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			<td class="xl65" height="21" style="height:15.75pt">BioSpec 117/16 magnetic resonance imaging system</td>
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			<td class="xl64" style="width:149pt" width="199">117/16</td>
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			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
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			<td class="xl65" height="21" style="height:15.75pt">Carbon steel scalpel no. 11/15</td>
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			<td class="xl65" height="21" style="height:15.75pt">Dressing forceps 115 mm&#160;</td>
			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">BD210R</td>
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		<tr height="21" style="height:15.75pt">
			<td class="xl65" height="21" style="height:15.75pt">Dressing forceps 130 mm&#160;</td>
			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">BD025R</td>
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			<td class="xl65" height="21" style="height:15.75pt">Frekaderm</td>
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			<td class="xl65" height="21" style="height:15.75pt">Gigli saw 0.44 mm&#160;</td>
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			<td class="xl64" style="width:149pt" width="199">RIS.590.110.25</td>
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			<td class="xl64" style="width:149pt" width="199">RIS.390.130-01</td>
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			<td class="xl64" style="width:149pt" width="199">2706</td>
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			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
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			<td class="xl65" height="21" style="height:15.75pt">Needle holder</td>
			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">BB078R</td>
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			<td class="xl65" height="21" style="height:15.75pt">Octenisept</td>
			<td class="xl65">Sch&#252;lke, Norderstedt, Germany</td>
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			<td class="xl65" height="21" style="height:15.75pt">Osirix software</td>
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			<td class="xl64" style="width:149pt" width="199">-</td>
			<td class="xl67">Version 4.0</td>
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			<td class="xl64" style="width:149pt" width="199">-</td>
			<td class="xl67"></td>
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		<tr height="21" style="height:15.75pt">
			<td class="xl65" height="21" style="height:15.75pt">Resolon 5/0</td>
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			<td class="xl64" style="width:149pt" width="199">88143</td>
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			<td class="xl64" style="width:149pt" width="199">3570350</td>
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			<td class="xl64" style="width:149pt" width="199">BB073R</td>
			<td class="xl67"></td>
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			<td class="xl64" style="width:149pt" width="199">BC006R</td>
			<td class="xl67"></td>
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			<td class="xl64" style="width:149pt" width="199">4657705</td>
			<td class="xl67"></td>
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		<tr height="21" style="height:15.75pt">
			<td class="xl65" height="21" style="height:15.75pt">Sterile surgical blades no. 11/15&#160;</td>
			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">BB511/515</td>
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			<td class="xl65" height="21" style="height:15.75pt">Surgical light&#160;</td>
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		<tr height="21" style="height:15.75pt">
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			<td class="xl64" style="width:149pt" width="199">Injekt 4606051V</td>
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			<td class="xl65" height="21" style="height:15.75pt">Tissue forceps 80 mm&#160;</td>
			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">OC091R</td>
			<td class="xl67"></td>
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		<tr height="21" style="height:15.75pt">
			<td class="xl65" height="21" style="height:15.75pt">Tramadol 25 mg/l</td>
			<td class="xl65">Gr&#252;nenthal, Aachen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">100mg/ml</td>
			<td class="xl67"></td>
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		<tr height="21" style="height:15.75pt">
			<td class="xl65" height="21" style="height:15.75pt">Vasofix Safety&#160;</td>
			<td class="xl65">Braun, Melsungen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">4268113S-01</td>
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			<td class="xl65" height="21" style="height:15.75pt">Vicryl 5-0&#160;</td>
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			<td class="xl64" style="width:149pt" width="199">V30371</td>
			<td class="xl67"></td>
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		<tr height="21" style="height:15.75pt">
			<td class="xl65" height="21" style="height:15.75pt">Visdisic eye ointment&#160;</td>
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			<td class="xl64" style="width:149pt" width="199">3099559</td>
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in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur

软骨骨折愈合是一个复杂的过程, 涉及发展的纤维, 软骨, 骨组织在骨折愈伤组织。骨痂中不同组织的数量为骨折愈合的进展提供了重要的信息。可用的体内技术纵向监测骨痂组织的发展在临床前骨折愈合研究中使用小动物包括数字造影和µCT 成像。然而, 这两种技术只能够区分矿化和 non-mineralized 组织。因此, 不可能区分软骨和纤维组织。相比之下, 磁共振成像 (MRI) 可视的解剖结构的基础上, 其含水量, 从而可以非识别软组织和软骨的骨折愈伤组织。在这里, 我们报告了使用 mri 兼容外固定器的小鼠股骨, 允许 mri 扫描在小鼠骨再生。实验表明, 该固定架和 custom-made 安装装置允许重复 MRI 扫描, 从而使纵向分析骨折-愈伤组织的发展。

首先, 通过对 MRI 扫描的分析可以确认手术的成功 (参见图 2中的示例)。所有四针应位于股骨干中间。截骨间隙的大小应介于 0.3-0.5 毫米之间。如果截骨间隙的大小与这些值相差很大, 则应将鼠标排除在进一步分析之外。
其次, 对同一动物骨折愈合过程中纵向扫描的评价提供了有关愈伤组织发育的信息。如?...

Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

在体内MRI 对小鼠股骨骨愈合过程中骨折愈伤组织发育的评价

软骨骨愈合过程中骨折愈伤组织发育的评价是监测愈合进程的关键。在这里, 我们报告的使用磁共振成像 (mri) 兼容外固定器的小鼠股骨, 允许 mri 扫描在小鼠骨再生。

Endochondral fracture healing is a complex process involving the development of fibrous, cartilaginous, and osseous tissue in the fracture callus. The ...

Research

JoVE Journal

Medicine

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

鼠标使用新型脊髓损伤的后肢骨质流失的纵向评价，在体内，方法论

Spinal cord injury (SCI) is often accompanied by osteoporosis in the sublesional regions of the pelvis and lower extremities, leading to a higher ...

科研

医学

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind

基于音频的虚拟游戏环境的发展，以帮助盲人的导航技巧

Audio-based Environment Simulator (AbES) is virtual environment software designed to improve real world navigation skills in the blind. Using only audio ...

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

顺序<em&gt;在体内</em&gt;成骨干/祖细胞在骨折修复的影像学

Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in ...

An Improved Method for Rapid Intubation of the Trachea in Mice

在小鼠气管插管快速的改进方法

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

An Intramedullary Locking Nail for Standardized Fixation of Femur Osteotomies to Analyze Normal and Defective Bone Healing in Mice

髓内锁钉股骨截骨标准化固定用于分析正常和有缺陷的骨愈合小鼠

Bone healing models are essential to the development of new therapeutic strategies for clinical fracture treatment. Furthermore, mouse models are becoming ...

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

伤口的牙髓评价一个直接盖髓模型的开发和治疗小鼠中修复性牙本质形成

Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is ...

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

用于评估椎弓根螺钉的麻醉，手术和收获方法<em&gt;体内</em&gt;猪腰椎模型

Pedicle screw fixation is the gold standard for the treatment of spinal diseases. However, many studies have reported the issue of loosening pedicle ...

A Minimally Invasive Model to Analyze Endochondral Fracture Healing in Mice Under Standardized Biomechanical Conditions

标准化生物力学条件下小鼠软骨骨折愈合的微创模型分析

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

小鼠闭合性股骨骨折的形成: 骨愈合模型的研究

Bone fractures impose a tremendous socio-economic burden on patients, in addition to significantly affecting their quality of life. Therapeutic strategies ...