Name: in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur
Uploaded: 2017-11-14T15:00:00
Description: Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

Vi takker Sevil Essig, Stefanie Schroth, Verena Fischer, Katja Prystaz, Yvonne Hägele og Anne Subgang for fremragende teknisk support. Vi takker også tyske Research Foundation (CRC1149, INST40/499-1) og Tysklands AO traumer fundament til finansiering af denne undersøgelse.

alle dyr eksperimenter overholdt internationale regler for pleje og anvendelse af forsøgsdyr og blev godkendt af de regionale tilsynsmyndigheder (No. 1250, Regierungspräsidium Tübingen, Tyskland). Alle mus blev opretholdt i grupper på to til fem dyr pr. bur på en 14-h lys, 10-h mørke døgnrytme med vand og mad leveret ad libitum.
 1. forberedelse af kirurgisk materiale og forbehandling af mus <ol><li>Sterilize alle kirurgiske materiale. Bruge en autoklavering temperatur på 1...

<ol>
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Ændringer og fejlfinding:
Hovedformålet med denne undersøgelse var at beskrive en protokol for ved hjælp af ekstern fiksator Mr-kompatible til lårbenet osteotomi i mus med evnen til at overvåge callus væv udvikling på langs under endochondral frakturheling processen. Design af en specialfremstillet monteringsudstyret til indsættelse af den ekstern fiksator sikret en standardiseret holdning under gentagne scanninger. Semi-automatiske væv segmentering giver mulighed fo...

Sekundære frakturheling er den mest almindelige form for knogleheling. Det er en kompleks proces efterligne specifikke aspekter af ontogenic endochondral ossifikation1,2,3. Den tidlige fraktur hæmatom består overvejende af immunceller, granulering og fibrøst væv. Lav ilt spænding og høje biomekaniske stammer hæmme osteoblastdannelse differentiering på fraktur gap, men fremme differentieringen af stamceller til chondrocytter4,5,6. Disse celler begynder at formere sig på stedet for skade at danne en bruskspidserne matrix giver indledende stabilitet af den brækkede knogle. Under callus modning, chondrocytter blive hypertrofisk, gennemgå apoptose, eller trans-differentiering til osteoblaster. Neovascularization på brusk til ben overgangszone giver forhøjet iltindhold, så dannelsen af benede væv7. Efter knoklet bridging fraktur Gap, biomekaniske stabilitet er øget og osteoclastic remodellering af eksterne fraktur hård hud opstår, for at få fysiologiske knogle omrids og struktur3. Derfor, mængder af fibrøst, bruskspidserne og knoklet væv i fraktur callus indeholder vigtige oplysninger om knoglen helingsproces. Forstyrret eller forsinket heling bliver synlig ved ændringer af hård hud væv udvikling både hos mennesker og mus8,9,10,11. Tilgængelige i vivo teknikker på langs overvåge callus væv udvikling i prækliniske fraktur healing undersøgelser ved hjælp af små dyr omfatter digital radiografi og µCT imaging12,13. Begge teknikker er imidlertid kun mulighed for at forskelsbehandle mineraliseret og ikke-mineraliseret væv. Derimod Mr giver fremragende bløddele kontrast og kunne derfor identificere blødt væv og brusk i fraktur callus.
Tidligere arbejde viste lovende resultater for post mortem Mr i mus med artikulære14 og i vivo Mr i mus under intramembranous knogle-defekt helbredende15. Begge undersøgelser erklærede imidlertid også begrænset rumlige opløsning og væv kontrast. Vi viste tidligere gennemførligheden af høj opløsning i vivo Mr langsgående vurdering af bløde callus dannelse under murine endochondral fraktur healing16. Her rapporterer vi protokollen for at anvende ekstern fiksator Mr-kompatible til lårbenet osteotomi i mus for at overvåge callus væv udvikling på langs under endochondral fraktur helingsproces. Design af en specialfremstillet monteringsudstyret til indsættelse af den ekstern fiksator sikret en standardiseret holdning under gentagne scanninger.

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			<td class="xl65" height="21" style="width:254pt;height:15.75pt" width="338">Anaesthesia tube</td>
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			<td class="xl64" style="width:149pt" width="199">117/16</td>
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			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
			<td class="xl64" style="width:149pt" width="199">BH 021R</td>
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			<td class="xl65" height="21" style="height:15.75pt">Carbon steel scalpel no. 11/15</td>
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			<td class="xl65">Aesculap, Tuttlingen, Germany</td>
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			<td class="xl64" style="width:149pt" width="199">-</td>
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			<td class="xl64" style="width:149pt" width="199">OC091R</td>
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			<td class="xl65" height="21" style="height:15.75pt">Tramadol 25 mg/l</td>
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			<td class="xl64" style="width:149pt" width="199">100mg/ml</td>
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in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur

Endochondral frakturheling er en kompleks proces, der omfatter udvikling af fibrøst, bruskspidserne og ossøse væv i fraktur callus. Mængden af de forskellige væv i callus indeholder vigtige oplysninger om fraktur healing fremskridt. Tilgængelige i vivo teknikker på langs overvåge callus væv udviklingen i prækliniske frakturheling studier ved hjælp af små dyr omfatter digital radiografi og µCT billeddannelse. Begge teknikker er imidlertid kun muligt at skelne mellem mineraliseret og ikke-mineraliseret væv. Det er derfor umuligt at diskriminere brusk fra fibrøst væv. I modsætning, magnetisk resonans imaging (MR) visualiserer anatomiske strukturer, der bygger på deres vandindhold og kunne derfor noninvasively identificere blødt væv og brusk i fraktur callus. Her rapporterer vi brugen af Mr-kompatibel ekstern fiksator for musen lårbenet tillade Mr-scanninger under knogle regenerering i mus. Forsøgene viste, at fiksator og en skræddersyet monteringsudstyret tillade gentagne Mr-scanninger, således at langsgående analyse af fraktur-callus væv udvikling.

Først, succesen af den kirurgiske procedure kan bekræftes ved analyse af Mr-scanninger (se eksemplet i figur 2). Alle fire ben skal være placeret i midten af femurdiafyse. Størrelsen af osteotomi afstanden skal være mellem 0,3-0,5 mm. Hvis størrelsen af osteotomi gap varierer meget fra disse værdier, bør musen udelukkes fra yderligere analyse.
For det andet indeholder evaluering af langsgåe...

Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

In Vivo Evaluering af fraktur Callus udvikling under knogleheling i mus ved hjælp af en Mr-kompatible osteosyntese enhed for musen lårben

Evaluering af væv udvikling i fraktur callus under endochondral knogleheling er afgørende at overvåge helingsprocessen. Her rapporterer vi brug af en magnetisk resonans imaging (MR)-kompatibel ekstern fiksator for musen lårbenet tillade MR scanner under knogle regenerering i mus.

Endochondral fracture healing is a complex process involving the development of fibrous, cartilaginous, and osseous tissue in the fracture callus. The ...

Research

JoVE Journal

Medicine

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Longitudinal Evaluering af Mouse Hind Limb knogletab Efter Rygmarvsskader med Roman, In vivo, Metodologi

Spinal cord injury (SCI) is often accompanied by osteoporosis in the sublesional regions of the pelvis and lower extremities, leading to a higher ...

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind

Udvikling af en Audio-based Virtual Gaming Miljø til at hjælpe med Navigation Skills i Blind

Audio-based Environment Simulator (AbES) is virtual environment software designed to improve real world navigation skills in the blind. Using only audio ...

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sekventiel<em&gt; In vivo</em&gt; Imaging af Osteogene Stem / stamceller Under frakturreparation

Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in ...

An Improved Method for Rapid Intubation of the Trachea in Mice

En forbedret fremgangsmåde til hurtig intubation af trachea i mus

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Udtømning af Mouse Celler fra Humane tumorxenoplantater Markant Forbedrer Downstream Analyse af Target Cells

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

An Intramedullary Locking Nail for Standardized Fixation of Femur Osteotomies to Analyze Normal and Defective Bone Healing in Mice

Et intramedullært Locking Nail for Standardiseret Fiksering af femur osteotomier at analysere Normal og Defekt Bone Healing i mus

Bone healing models are essential to the development of new therapeutic strategies for clinical fracture treatment. Furthermore, mouse models are becoming ...

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Udvikling af en Direct Pulp-capping Model for Vurdering af pulpa Sårheling og Reparative Dentin Formation i mus

Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is ...

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

En bedøvelses-, kirurgisk og høstmetode til evaluering af transpedikulære skruer ved anvendelse af en<em&gt; In vivo</em&gt; Porcine Lumbar Spine Model

Pedicle screw fixation is the gold standard for the treatment of spinal diseases. However, many studies have reported the issue of loosening pedicle ...

A Minimally Invasive Model to Analyze Endochondral Fracture Healing in Mice Under Standardized Biomechanical Conditions

En minimalt invasiv Model til at analysere Endochondral fraktur Healing i mus Under standardiserede biomekaniske forhold

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Generation af lukkede femur frakturer i mus: en Model til at studere knogleheling

Bone fractures impose a tremendous socio-economic burden on patients, in addition to significantly affecting their quality of life. Therapeutic strategies ...