Name: in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur
Uploaded: 2017-11-14T15:00:00
Description: Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

Vi takker Sevil Essig, Stefanie Schroth, Verena Fischer, Katja Prystaz, Yvonne Hägele og Anne Subgang utmerket kundestøtte. Vi takker også tysk Research Foundation (CRC1149, INST40/499-1) og AO traumer Foundation Tyskland for finansiering denne studien.

alle dyr eksperimenter overholdt internasjonale regler og bruk av forsøksdyr og ble godkjent av de regionale myndighetene (nr. 1250, Regierungspräsidium Tübingen i Tyskland). Alle mus ble opprettholdt i grupper på to til fem dyr per bur på 14-h lys, 10-h mørke døgnrytmen med vann og mat gitt annonse libitum.
 1. forberedelse av kirurgiske materiale og forbehandling av mus <ol><li>Sterilize alle kirurgiske materiale. Bruke en autoklavering temperatur på 120-135 ° C for 20-3...

<ol>
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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Age+and+ovariectomy+impair+both+the+normalization+of+mechanical+properties+and+the+accretion+of+mineral+by+the+fracture+callus+in+rats.">Age and ovariectomy impair both the normalization of mechanical properties and the accretion of mineral by the fracture callus in rats.</a> J Orthop Res. 19 (3), 428-435 (2001).</li><li>Nikolaou, V. S., Efstathopoulos, N., Kontakis, G., Kanakaris, N. K., Giannoudis, P. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+influence+of+osteoporosis+in+femoral+fracture+healing+time.">The influence of osteoporosis in femoral fracture healing time.</a> Injury. 40 (6), 663-668 (2009).</li><li>Haffner-Luntzer, M., Kovtun, A., Rapp, A. 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M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ImmunoPET/MR+imaging+allows+specific+detection+of+Aspergillus+fumigatus+lung+infection+in+vivo.">ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.</a> Proc Natl Acad Sci U S A. 113 (8), 1026-1033 (2016).</li><li>Niemeyer, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+tracking+of+human+haemopoietic+CD34(+)+stem+cells+in+vivo+in+immunodeficient+mice+by+using+magnetic+resonance+imaging.">Non-invasive tracking of human haemopoietic CD34(+) stem cells in vivo in immunodeficient mice by using magnetic resonance imaging.</a> Eur Radiol. 20 (9), 2184-2193 (2010).</li></ol>

Endringer og feilsøking:
Hovedmålet med denne studien var å beskrive en protokoll for bruk av en MRI-kompatibel ekstern fixator for femur osteotomi musen muligheten til å overvåke callus vev utvikling langs under endochondral brudd-healing prosessen. Utformingen av en skreddersydd montering enhet for innsetting av den eksterne fixator sikret en standardisert posisjon under gjentatt skanner. Halvautomatisk vev segmentering tillater analyse av mengder fibrøse cartilaginous...

Sekundær brudd healing er den vanligste formen for bein helbredelse. Det er en kompleks prosess mimicking bestemte aspekter av ontogenic endochondral forbening1,2,3. Tidlig brudd hematom består hovedsakelig av immunceller, korning og bindevev. Oksygen spenning og høy biomekaniske stammer hemme osteoblast differensieringen brudd gapet, men fremme differensiering av progenitor celler i chondrocytes4,5,6. Disse cellene begynner å spre seg på stedet av skade å danne en cartilaginous matrise gir første stabiliteten i fractured benet. Under callus modning, chondrocytes bli hypertrofisk, gjennomgår apoptose, eller trans-skille ut osteoblasts. Neovascularization på brusk-til-Ben overgang zone gir forhøyet oksygen nivåer, slik at dannelsen av bein vev7. Etter benete bygge bro av brudd gapet, biomekaniske stabilitet er økt og osteoclastic ombygging av eksterne brudd callus oppstår for å få fysiologiske bein kontur og struktur3. Derfor inneholder mengder fibrøse cartilaginous og bein vev i brudd callus viktig informasjon om benet helbredelsesprosessen. Forstyrret eller forsinket healing blir synlig ved endringer callus vev utvikling både mennesker og mus8,9,10,11. Tilgjengelig i vivo teknikker langs overvåke callus vev utvikling i preklinisk brudd healing studier med liten dyrene inkluderer digital røntgen og µCT imaging12,13. Men kan begge teknikkene bare forskjellsbehandle mineralholdig og ikke-mineraliserte vev. I kontrast, Mr gir utmerket bløtvev kontrast og derfor kunne identifisere mykt vev og brusk i brudd callus.
Tidligere arbeid viste lovende resultater for post mortem MRI i mus med articular frakturer14 og i vivo MRI i mus under intramembranous bein-defekt helbredende15. Men uttalt både studier også begrenset romlig oppløsning og vev kontrast. Vi viste tidligere muligheten for høy oppløsning i vivo MRI langsgående vurdering av myke callus formasjon under murine endochondral brudd healing16. Her rapportere vi av protokollen for å bruke en MRI-kompatibel ekstern fixator for femur osteotomi i mus for å overvåke callus vev utvikling langs under endochondral brudd helbredelsesprosessen. Utformingen av en skreddersydd montering enhet for innsetting av den eksterne fixator sikret en standardisert posisjon under gjentatt skanner.

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in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur

Endochondral brudd healing er en kompleks prosess som involverer utviklingen av fibrøst cartilaginous og osseous vev i brudd callus. Mengden av ulike vev i callus gir viktig informasjon om brudd healing fremgang. Tilgjengelig i vivo teknikker å overvåke langs callus vev utviklingen i preklinisk brudd-healing studier med liten dyrene inkluderer digital røntgen og µCT bildebehandling. Begge teknikkene er imidlertid bare kunne skille mellom mineralholdig og ikke-mineraliserte vev. Derfor er det umulig å diskriminere brusk fra fibrøst vev. I kontrast, magnetisk resonans imaging (MRI) visualiserer anatomiske strukturer basert på sitt vanninnhold og derfor kunne identifisere noninvasively mykt vev og brusk i brudd callus. Her rapporterer vi bruk av en MRI-kompatibel ekstern fixator for musen femur at Mr skanner under bein regenerasjon i mus. Eksperimenter har vist at fixator og en skreddersydd montering tillater repeterende Mr skanner, dermed muliggjør langsgående analyse av brudd-callus vev utvikling.

Først kan suksessen til den kirurgiske prosedyren bekreftes ved analyse av Mr skanner (se eksemplet i figur 2). Alle de fire trådene bør være plassert midt i femur akselen. Størrelsen på osteotomi gapet bør være mellom 0,3 til 0.5 mm. Hvis størrelsen på osteotomi gapet varierer mye fra disse verdiene, bør musen bli ekskludert fra videre analyse.
Dernest gir evalueringen av langsgående sk...

Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

I Vivo Evaluering av brudd Callus utvikling i bein Healing i mus bruker en MRI-kompatibel Osteosynthesis enhet for musen Femur

Evalueringen av vev utvikling i brudd callus under endochondral bein healing er viktig å overvåke helbredelsesprosessen. Her rapporterer vi bruk av en magnetisk resonans imaging (MRI)-kompatibel ekstern fixator for musen femur tillate MRI finkjemmer bein gjenfødelse i mus.

Endochondral fracture healing is a complex process involving the development of fibrous, cartilaginous, and osseous tissue in the fracture callus. The ...

Research

JoVE Journal

Medicine

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Langsgående Evaluering av Mouse Hind Limb Bone tap etter ryggmargsskade bruke Novel, In vivo Methodology

Spinal cord injury (SCI) is often accompanied by osteoporosis in the sublesional regions of the pelvis and lower extremities, leading to a higher ...

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind

Utvikling av en Audio-baserte Virtual Gaming Environment for å bistå med Navigasjon Skills i Blind

Audio-based Environment Simulator (AbES) is virtual environment software designed to improve real world navigation skills in the blind. Using only audio ...

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sekvensiell<em&gt; In vivo</em&gt; Imaging av osteogent Stem / stamceller under brudd Repair

Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in ...

An Improved Method for Rapid Intubation of the Trachea in Mice

En forbedret fremgangsmåte for Rapid intubering av trakea hos mus

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Nedbryting av museceller fra menneske tumorxenotransplantater forbedrer Nedstrøms Analyse av Target Cells

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

An Intramedullary Locking Nail for Standardized Fixation of Femur Osteotomies to Analyze Normal and Defective Bone Healing in Mice

En Intramedullære Låse Nail for Standardisert Fiksering av Femur osteotomier å analysere Normal og Defekt Bone Healing i Mus

Bone healing models are essential to the development of new therapeutic strategies for clinical fracture treatment. Furthermore, mouse models are becoming ...

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Utvikling av en direkte Pulp-capping modell for evaluering av pulpal Wound Healing og reparerende Dentin Dannelse i Mus

Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is ...

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

En bedøvelses-, kirurgisk og høstmetode for evaluering av transpedikulære skruer ved hjelp av en<em&gt; In vivo</em&gt; Porcine Lumbar Spine Model

Pedicle screw fixation is the gold standard for the treatment of spinal diseases. However, many studies have reported the issue of loosening pedicle ...

A Minimally Invasive Model to Analyze Endochondral Fracture Healing in Mice Under Standardized Biomechanical Conditions

En minimal invasiv modell for å analysere Endochondral brudd i mus Under standardisert biomekaniske forhold

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Generering av lukkede femur brudd i mus: en modell for å studere bein Healing

Bone fractures impose a tremendous socio-economic burden on patients, in addition to significantly affecting their quality of life. Therapeutic strategies ...