Name: in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur
Uploaded: 2017-11-14T15:00:00
Description: Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

Sevil Essig, Stefanie Schroth, Verena Fischer, Katja Prystaz, Yvonne Hägele ve Anne Subgang mükemmel teknik destek için teşekkür ederiz. Ayrıca bu çalışmada finansmanı için AO travma Vakfı Almanya ve Alman Araştırma Vakfı (CRC1149, INST40/499-1) teşekkür ederiz.

tüm hayvan deneyleri için bakım ve kullanım laboratuvar hayvanlarının uluslararası düzenlemelere uyulması ve bölgesel düzenleyici otoriteler tarafından (No 1250, Regierungspräsidium Tübingen, Almanya) kabul edildi. Bütün fareler yapılmaktadır 14-h ateşin üzerinde kafes başına iki ila beş hayvanların gruplarındaki 10-h karanlık sirkadiyen ritim su ve yiyecek ile sağlanan ad libitum.
 1. cerrahi malzeme ve farelerin ön arıtma hazırlanması <ol><li>Steril...

<ol>
	<li>Claes, L., Recknagel, S., Ignatius, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fracture+healing+under+healthy+and+inflammatory+conditions.">Fracture healing under healthy and inflammatory conditions.</a> Nat Rev Rheumatol. 8 (3), 133-143 (2012).</li><li>Einhorn, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+cell+and+molecular+biology+of+fracture+healing.">The cell and molecular biology of fracture healing.</a> Clin Orthop Relat Res. (355), S7-S21 (1998).</li><li>Einhorn, T. A., Gerstenfeld, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fracture+healing:+mechanisms+and+interventions.">Fracture healing: mechanisms and interventions.</a> Nat Rev Rheumatol. 11 (1), 45-54 (2015).</li><li>Augat, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+tissue+properties+in+bone+healing:+influence+of+size+and+stability+of+the+osteotomy+gap.">Local tissue properties in bone healing: influence of size and stability of the osteotomy gap.</a> J Orthop Res. 16 (4), 475-481 (1998).</li><li>Claes, L. E., Heigele, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnitudes+of+local+stress+and+strain+along+bony+surfaces+predict+the+course+and+type+of+fracture+healing.">Magnitudes of local stress and strain along bony surfaces predict the course and type of fracture healing.</a> J Biomech. 32 (3), 255-266 (1999).</li><li>Claes, L. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+mechanical+factors+on+the+fracture+healing+process.">Effects of mechanical factors on the fracture healing process.</a> Clin Orthop Relat Res. (355), 132-147 (1998).</li><li>Hu, D. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cartilage+to+bone+transformation+during+fracture+healing+is+coordinated+by+the+invading+vasculature+and+induction+of+the+core+pluripotency+genes.">Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes.</a> Development. 144 (2), 221-234 (2017).</li><li>Hankenson, K. D., Zimmerman, G., Marcucio, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+perspectives+of+delayed+fracture+healing.">Biological perspectives of delayed fracture healing.</a> Injury. 45, 8-15 (2014).</li><li>Meyer, R. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Age+and+ovariectomy+impair+both+the+normalization+of+mechanical+properties+and+the+accretion+of+mineral+by+the+fracture+callus+in+rats.">Age and ovariectomy impair both the normalization of mechanical properties and the accretion of mineral by the fracture callus in rats.</a> J Orthop Res. 19 (3), 428-435 (2001).</li><li>Nikolaou, V. S., Efstathopoulos, N., Kontakis, G., Kanakaris, N. K., Giannoudis, P. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+influence+of+osteoporosis+in+femoral+fracture+healing+time.">The influence of osteoporosis in femoral fracture healing time.</a> Injury. 40 (6), 663-668 (2009).</li><li>Haffner-Luntzer, M., Kovtun, A., Rapp, A. E., Ignatius, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+Models+in+Bone+Fracture+Healing+Research.">Mouse Models in Bone Fracture Healing Research.</a> Current Molecular Biology Reports. 2 (2), 101-111 (2016).</li><li>Garcia, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rodent+animal+models+of+delayed+bone+healing+and+non-union+formation:+a+comprehensive+review.">Rodent animal models of delayed bone healing and non-union formation: a comprehensive review.</a> Eur Cell Mater. 26, 1-14 (2013).</li><li>Histing, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+animal+bone+healing+models:+standards,+tips,+and+pitfalls+results+of+a+consensus+meeting.">Small animal bone healing models: standards, tips, and pitfalls results of a consensus meeting.</a> Bone. 49 (4), 591-599 (2011).</li><li>Zachos, T. A., Bertone, A. L., Wassenaar, P. A., Weisbrode, S. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rodent+models+for+the+study+of+articular+fracture+healing.">Rodent models for the study of articular fracture healing.</a> J Invest Surg. 20 (2), 87-95 (2007).</li><li>Taha, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+the+efficacy+of+MRI+for+detection+of+changes+in+bone+morphology+in+a+mouse+model+of+bone+injury.">Assessment of the efficacy of MRI for detection of changes in bone morphology in a mouse model of bone injury.</a> J Magn Reson Imaging. 38 (1), 231-237 (2013).</li><li>Haffner-Luntzer, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+high-resolution+In+Vivo+MRI+for+longitudinal+analysis+of+endochondral+fracture+healing+in+mice.">Evaluation of high-resolution In Vivo MRI for longitudinal analysis of endochondral fracture healing in mice.</a> PLoS One. 12 (3), 0174283 (2017).</li><li>Beckmann, N., Falk, R., Zurbrugg, S., Dawson, J., Engelhardt, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Macrophage+infiltration+into+the+rat+knee+detected+by+MRI+in+a+model+of+antigen-induced+arthritis.">Macrophage infiltration into the rat knee detected by MRI in a model of antigen-induced arthritis.</a> Magn Reson Med. 49 (6), 1047-1055 (2003).</li><li>Al Faraj, ., Shaik A, S. u. l. t. a. n. a., Pureza, A., A, M., Alnafea, M., Halwani, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preferential+macrophage+recruitment+and+polarization+in+LPS-induced+animal+model+for+COPD:+noninvasive+tracking+using+MRI.">Preferential macrophage recruitment and polarization in LPS-induced animal model for COPD: noninvasive tracking using MRI.</a> PLoS One. 9 (3), 90829 (2014).</li><li>Rolle, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ImmunoPET/MR+imaging+allows+specific+detection+of+Aspergillus+fumigatus+lung+infection+in+vivo.">ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.</a> Proc Natl Acad Sci U S A. 113 (8), 1026-1033 (2016).</li><li>Niemeyer, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+tracking+of+human+haemopoietic+CD34(+)+stem+cells+in+vivo+in+immunodeficient+mice+by+using+magnetic+resonance+imaging.">Non-invasive tracking of human haemopoietic CD34(+) stem cells in vivo in immunodeficient mice by using magnetic resonance imaging.</a> Eur Radiol. 20 (9), 2184-2193 (2010).</li></ol>

Değişiklikler ve sorun giderme:
Kallus dokusu geliştirme boyuna endochondral kırık iyileşmesi işlemi sırasında izleme olanağı ile uyluk osteotomi fare için bir MRI uyumlu eksternal fiksatör ile kullanmak için bir iletişim kuralı tanımlamak için bu çalışmanın ana hedefi oldu. Eksternal fiksatör yerleştirilmesi için özel montaj aygıtının tasarım standart bir pozisyon tekrarlanan taramalar sırasında sağlamıştır. Yarı otomatik doku segmentasyon...

İkincil kırık iyileşmesi kemik iyileşme en yaygın şeklidir. Ontogenic endochondral ossifikasyon1,2,3, belirli özellikleriyle taklit karmaşık bir süreçtir. Erken kırık hematom ağırlıklı olarak bağışıklık hücreleri, granülasyon ve fibröz doku oluşur. Düşük oksijen gerilim ve yüksek biyomekanik suşları osteoblast farklılaşma kırık boşluğu, engel ama progenitör hücre farklılaşma kondrosit4,5,6içine teşvik. Bu hücreler kırık kemiğin ilk istikrar sağlayan bir kıkırdak matris oluşturmak için yaralanma sitesinde çoğalırlar başlar. Kallus olgunlaşma sırasında kondrosit Hipertrofik, haline geçmesi apoptosis veya trans-dokusunu ayırt etmek. Neovaskülarizasyon kıkırdak-kemik geçiş bölgesi, yüksek oksijen düzeyleri, kemik doku7oluşumunu sağlayarak verir. Kemik kırığı köprü sonra daha biyomekanik istikrarlı ve fizyolojik kemik kontur ve yapısı3kazanmak için sebep harici kırığı Kallus remodeling oluşur. Bu nedenle, kırık Kallus fibröz kıkırdak ve kemik dokusunda miktarda kemik iyileşme süreci hakkında önemli bilgiler sağlar. Rahatsız veya gecikmeli şifa değişiklikler Kallus dokusu gelişme hem insanlar ve fareler8,9,10,11tarafından görünür hale gelir. Dijital Radyografi ve12,13görüntüleme µCT boyuna preklinik kırık küçük hayvanlar kullanarak çalışmalar şifa Nasırlaşma doku gelişiminde izlemek için kullanılabilir vivo içinde teknikleri içerir. Ancak, her iki teknikleri sadece mineralize ve mineralize olmayan doku arasında ayırımcılık edebiliyoruz. Buna ek olarak, Mr mükemmel yumuşak doku kontrast sağlar ve bu nedenle yumuşak doku ve kıkırdak içinde kırık Kallus tanımlamak mümkün olabilir.
Önceki çalışma Intramembranous kemik-kusur şifa15sırasında farelerde post mortem MRI farelerde ile eklem kırıkları14 ve in vivo MRI için umut verici sonuçlar gösterdi. Bununla birlikte, her iki çalışmaları da sınırlı uzamsal çözünürlük ve doku kontrast belirtti. Biz daha önce yüksek çözünürlüklü vivo içinde Mr16şifa fare endochondral kırık sırasında yumuşak Kallus oluşumu boyuna değerlendirmesi için fizibilite gösterdi. Burada, Kallus dokusu geliştirme boyuna endochondral kırık iyileşme süreci sırasında izlemek için uyluk osteotomi farelerde için bir MRI uyumlu eksternal fiksatör kullanarak için protokol raporu. Eksternal fiksatör yerleştirilmesi için özel montaj aygıtının tasarım standart bir pozisyon tekrarlanan taramalar sırasında sağlamıştır.

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in vivo evaluation of fracture callus development during bone healing in mice using an mri-compatible osteosynthesis device for the mouse femur

Endochondral kırık iyileşmesi fibröz kıkırdak ve kemik dokusunda kırık Kallus gelişimi içeren karmaşık bir işlemdir. Kallus farklı dokularda miktarda ilerleme şifa kırık önemli bilgiler sağlar. Dijital Radyografi ve µCT görüntüleme boyuna preklinik kırık iyileşmesi çalışmalar küçük hayvanlar kullanarak Kallus dokusu gelişiminde izlemek için kullanılabilir vivo içinde teknikleri içerir. Ancak, her iki teknikleri sadece mineralize ve mineralize olmayan doku arasında ayırt edebiliyoruz. Sonuç olarak, kıkırdak fibröz doku ayırt etmek mümkün değildir. Buna ek olarak, manyetik rezonans görüntüleme (MRG) su içeriğine göre anatomik yapıları görüntüler ve bu nedenle Noninvazif yumuşak doku ve kıkırdak içinde kırık Kallus tanımlamak mümkün olabilir. Burada, Mr uyumlu fare uyluk için fiksatör MRI taramaları farelerde kemik yeniden oluşturma işlemi sırasında izin vermek için dış kullanımı raporu Deneyler fiksatör ve ısmarlama montaj aygıt tekrarlayan MRI taramaları, böylece kırık-Kallus dokusu gelişimin boyuna analizi etkinleştirmek izin gösterdi.

İlk olarak, cerrahi işlem başarısı analizi ( Şekil 2' deki örneğe bakın) MRI taramaları tarafından onaylanabilir. Tüm dört iğne ortasında femur şaft yerleştirilmemesi gerekir. Osteotomi boşluk boyutu 0.3-0.5 arasında olmalıdır mm. Osteotomi boşluk boyutunu büyük ölçüde bu değerler değişiyorsa, fare daha fazla analiz çıkarılmalıdır.
İkinci olarak, boyuna taramalar?...

Watch this Scientific Journal Video about In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur at JoVE.com

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Vivo Fare uyluk için bir MRI uyumlu Osteosentez aygıtı kullanma farelerde kemik iyileşme sırasında kırık Kallus gelişimi değerlendirme

Endochondral kemik iyileşme sırasında kırık Kallus dokusu geliştirme değerlendirilmesi iyileşme süreci izlemek için önemlidir. Burada, bir manyetik rezonans görüntüleme (MRG) kullanımı raporu-uyumlu eksternal fiksatör fare uyluk için MRG izin farelerde kemik rejenerasyon sırasında tarar.

Endochondral fracture healing is a complex process involving the development of fibrous, cartilaginous, and osseous tissue in the fracture callus. The ...

Research

JoVE Journal

Medicine

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Roman kullanarak Spinal Kord Yaralanması sonra Fare Hind Ekstremite Kemik Kaybı Boyuna değerlendirilmesi, In vivo, Metodolojisi

Spinal cord injury (SCI) is often accompanied by osteoporosis in the sublesional regions of the pelvis and lower extremities, leading to a higher ...

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind

Ses tabanlı Sanal Oyun Ortamı Geliştirilmesi Kör Navigasyon Becerileri ile Yrd etmek

Audio-based Environment Simulator (AbES) is virtual environment software designed to improve real world navigation skills in the blind. Using only audio ...

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Ardışık<em&gt; In vivo</em&gt; Kırık Onarım sırasında Osteojenik Kök / Progenitör Hücrelerinin Görüntüleme

Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in ...

An Improved Method for Rapid Intubation of the Trachea in Mice

Farelerde Trakea hızlı intübasyon için geliştirilmiş bir yöntem

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

İnsan Tümör ksenogratflarının gelen Fare Hücrelerinin tükenmesi Anlamlı Hedef Hücre Alt Analizi geliştirir

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

An Intramedullary Locking Nail for Standardized Fixation of Femur Osteotomies to Analyze Normal and Defective Bone Healing in Mice

Femur Osteotomiler standardize Sabitleme için bir intramedüller Kilitleme Nail Fare Normal ve Arızalı Kemik Şifa Analiz

Bone healing models are essential to the development of new therapeutic strategies for clinical fracture treatment. Furthermore, mouse models are becoming ...

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Pulpa Değerlendirilmesi için Doğrudan Selüloz-kapatma Modelinin Geliştirilmesi Yara İyileşmesi ve Farelerde Onarım dentin oluşumu

Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is ...

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

Transpediküler Vidaların Değerlendirilmesinde Anestezi, Cerrahi ve Hasat Yöntemi<emIn vivo</em&gt; Domuz Kemik Omurgası Modeli

Pedicle screw fixation is the gold standard for the treatment of spinal diseases. However, many studies have reported the issue of loosening pedicle ...

A Minimally Invasive Model to Analyze Endochondral Fracture Healing in Mice Under Standardized Biomechanical Conditions

Endochondral standart biyomekanik koşullar altında farelerde şifa kırık çözümlemek için minimal invaziv bir Model

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Farelerde kapalı femur kırığı nesil: kemik şifa çalışması için bir Model

Bone fractures impose a tremendous socio-economic burden on patients, in addition to significantly affecting their quality of life. Therapeutic strategies ...