Name: intraperitoneal glucose tolerance test, measurement of lung function, and fixation of the lung to study the impact of obesity and impaired metabolism on pulmonary outcomes
Uploaded: 2018-03-15T13:00:00
Description: Watch this Scientific Journal Video about Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmo

実験に支えられたマルガとウォルター ・ ボル ・財団、ドイツ;210-02-16 (MAAA) 210-03-15 (MAAA) プロジェクトをプロジェクト、ドイツの研究基礎 (DFG;AL1632-02MAAA)、ボン、ドイツ;分子医学ケルン (CMMC; の中心大学病院ケルン;キャリアの進歩プログラム;MAAA) フォーチュン ケルン (ケルンの大学の医学部KD)。

動物のすべてのプロシージャは地方自治体によって承認されたプロトコルに準拠して実施された (nrw 州、アリゾナ州の土地: 2012.A424)、ドイツの動物福祉法および、実験のために使用される動物の福祉に関する法令に従い、他の科学的な目的。肺機能解析肺構造に影響を与える可能性がありますので、したがってそれに続く組織学的分析解像度と強度のため肺の固定と Cdyn 準備の測定は異なっ?...

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このレポートでは、糖代謝、呼吸器アウトカムに対する肥満の影響を分析する 3 つの異なる方法の 3 つのプロトコルを提供します。まず、ブドウ糖負荷試験はグルコースの細胞内取り込みを分析する機会を提供しています、インスリン抵抗性を示すことができます。第二に、全身脈は肺機能を測定する技術、それにより新しい治療法の有効性をテストすると便利です。第三に、標準化された?...

よると、世界保健機関 (WHO) 2008 年に、20 歳以上、以上 14 億の大人たち、ボディマス指数 (BMI); 25 以上を太りすぎさらに、以上 2 億人、ほぼ 3 億女性肥満 (BMI≥30)1。肥満やメタボリック シンドロームは、多数の病気のための主要な危険因子です。肥満と併用の白色脂肪組織増加中質量密接にリンクされているタイプ 2 糖尿病2,3, 冠状動脈性心臓病 (CHD)、心不全 (心不全)、心房細動4 を含む心臓血管疾患変形性関節症5、呼吸器系疾患の病態における機能的な役割のままかり。ただし、疫学的研究は、肥満が労作時呼吸困難、睡眠時無呼吸症候群 (OSAS) 肥満低換気症候群 (OHS) 慢性を含む慢性の呼吸器の条件に強く関連付けられているを示しています。閉塞性肺疾患 (COPD)、肺塞栓症、誤嚥性肺炎、気管支喘息6,7,8,9。リンク肥満と摂動代謝潜在的なメカニズム、例えば、インスリン抵抗性、タイプ II 糖尿病は、慢性肺疾患の病態だけでなく構成重量の機械および物理的な結果も換気益亜急性炎症状態が慢性化10,11を誘発します。肥満と効果的な予防戦略と治療アプローチの欠如と相まって、過去 10 年間の間に肺疾患の増加肥満関連肺を管理するための新しい道を定義する分子メカニズム解明のための必要性を強調します。病気。
ここでは、肥満とマウス モデルにおける肺の構造と機能への影響を調査するための重要な基礎である 3 つの標準的なテストについて述べる: (1) 腹腔内グルコース耐性 (ipGTT) (2) 気道抵抗 (Re) の測定と呼吸システム コンプライアンス (Cdyn);(3) の作製とその後定量組織学的評価のため肺の固定。IpGTT は、メジャー グルコース取り込みと代謝に及ぼす肥満への堅牢なスクリーニング テストです。方法のシンプルさは、研究所間標準化、その結果の比較可能性をできます。代謝表現型12の詳細な分析、血糖クランプや膵島の研究より高度な方法を使用できます。ここでは、肺の結果にさらなる研究のための基礎として全身性および代謝障害の肥満準の状態を定義する耐糖能を評価します。肥満と代謝性疾患の肺機能に及ぼす影響を評価するためには、気道抵抗 (Re) と呼吸器系コンプライアンス (Cdyn) を測定しました。肺疾患の特徴、気ままなとして拘束された肺機能の評価手法も利用できます。動物を自由に移動で気ままな脈波呼吸パターンを反映して、自然な状態を模倣しています。対照的に、動的肺力学を評価するために解像度、および深く麻酔下マウスにおける cDyn の入力インピー ダンス測定などの侵襲的な方法より正確な13です。慢性の呼吸器疾患が肺組織の組織学的変化が反映されているので、さらなる分析のための適切な肺固定が迫っています。ティッシュの固定および準備の方法の選択は、勉強になります、例えば、気道や肺実質14肺のコンパートメントに依存します。ここでは、喘息発症に対する肥満の影響を研究する導電性の気道の定性的および定量的な評価を可能にする手法について述べる。

<table>
	<tbody>
		<tr>
			<td>GlucoMen LX</td>
			<td>A.Menarini diagnostics, Firneze, Italy</td>
			<td>38969</td>
			<td>blood glucose meter</td>
		</tr>
		<tr>
			<td>GlucoMen LX Sensor</td>
			<td>A.Menarini diagnostics, Firneze, Italy</td>
			<td>39765</td>
			<td>Test stripes</td>
		</tr>
		<tr>
			<td>Glucose 20%</td>
			<td>B. Braun, Melsung, Germany</td>
			<td>2356746</td>
			<td></td>
		</tr>
		<tr>
			<td>FinePointe Software</td>
			<td>DSI, MC s&#180;Hertogenbosch, Netherlands</td>
			<td>601-1831-002</td>
			<td></td>
		</tr>
		<tr>
			<td>FinePointe RC Single Site Mouse Table</td>
			<td>DSI, MC s&#180;Hertogenbosch, Netherlands</td>
			<td>601-1831-001</td>
			<td></td>
		</tr>
		<tr>
			<td>FPRC Controller</td>
			<td>DSI, MC s&#180;Hertogenbosch, Netherlands</td>
			<td>601-1075-001</td>
			<td></td>
		</tr>
		<tr>
			<td>FPRC Aerosol Block</td>
			<td>DSI, MC s&#180;Hertogenbosch, Netherlands</td>
			<td>601-1106-001</td>
			<td></td>
		</tr>
		<tr>
			<td>Aerogen neb head-5.2-4um</td>
			<td>DSI, MC s&#180;Hertogenbosch, Netherlands</td>
			<td>601-2306-001</td>
			<td></td>
		</tr>
		<tr>
			<td>Forceps</td>
			<td>FST, British Columbia, Canada</td>
			<td>11065-07</td>
			<td></td>
		</tr>
		<tr>
			<td>Blunt scissors</td>
			<td>FST, British Columbia, Canada</td>
			<td>14105-12</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro scissors</td>
			<td>FST, British Columbia, Canada</td>
			<td>15000-00</td>
			<td></td>
		</tr>
		<tr>
			<td>Perma-Hand 4-0</td>
			<td>Ethicon, Puerto Rico, USA</td>
			<td>736H</td>
			<td>Surgical suture</td>
		</tr>
		<tr>
			<td>Roti-Histofix 4%</td>
			<td>Roth</td>
			<td>P087.1</td>
			<td>4% Paraformaldehyd</td>
		</tr>
		<tr>
			<td>Ketaset</td>
			<td>Zoetis, Berlin, Germany</td>
			<td>10013389</td>
			<td>Ketamine</td>
		</tr>
		<tr>
			<td>Rompun 2%</td>
			<td>Bayer, Leverkusen, Germany</td>
			<td>770081</td>
			<td>Xylazine</td>
		</tr>
	</tbody>
</table>

intraperitoneal glucose tolerance test, measurement of lung function, and fixation of the lung to study the impact of obesity and impaired metabolism on pulmonary outcomes

肥満と呼吸器系疾患は、主要な健康上の問題です。肥満は、こうして社会経済的負担の増大を表す 2030 以上 10 億肥満の個人の世界的な数が期待される新たな流行をなっています。同時に、肥満関連の合併症、糖尿病と同様に心や慢性肺疾患などが継続的に増加しています。肥満は喘息の増悪のリスクの増加に関連付けられている、呼吸器症状、およびコントロール不良の悪化肥満や慢性肺疾患の発症機序における摂動代謝の機能的役割がしばしば過小評価、その分子メカニズムのとらえどころのないままです。この記事は、代謝と同様に肺の構造と機能に対する肥満の影響を評価する手法を提示する目指しています。ここでは、マウスの調査の 3 つの技術について述べる: 腹腔内グルコース耐性 (ipGTT); 糖代謝に対する肥満の影響を分析するための (1) の評価(気道抵抗 (Re) と呼吸器系コンプライアンス (Cdyn) 肺機能に対する肥満の影響を分析するための測定 2)(3) の作製とその後定量組織学的評価のため肺の固定。肥満関連肺疾患は、おそらく、潜在的悪影響肺機能および治療への反応に影響を与える全身の炎症と代謝不全から生じる多因子性。したがって、分子メカニズムと新しい治療法の効果を検討する標準化された方法論が不可欠です。

腹腔内のブドウ糖負荷試験 (ipGTT) (図 4) の代表的な結果は、ヘマトキシリンとエオシンを示す肺関数テスト (図 5)、および代表画像ステンド肺 (図 6)。
IpGTT は、高脂肪食 (HFD) の 7 週間後 (青) 肥満のマウスで行われました。標準的な食事供給のマウス コン?...

Watch this Scientific Journal Video about Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmo

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

肥満の発生率が上昇していると慢性肺疾患のリスクを増加させます。根本的なメカニズムと予防戦略、明確に定義された動物を確立するには、モデルが必要です。ここでは、我々 は (ブドウ糖負荷試験、体の脈、肺固定) マウスにおける肺の成果に対する肥満の影響を研究する 3 つの方法を提供します。

腹腔内のブドウ糖負荷試験、肥満や呼吸器アウトカムに対する代謝が異常の影響を研究する肺機能の測定や肺の固定

Obesity and respiratory disorders are major health problems. Obesity is becoming an emerging epidemic with an expected number of over 1 billion obese ...

Research

JoVE Journal

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There  is growing concern about the relevance of in vitro antimicrobial  susceptibility tests when applied to isolates of P. aeruginosa from  cystic ...

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隔離された心筋細胞の細胞機能に対する抗体の効果測定

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Right Ventricular Systolic Pressure Measurements in Combination with Harvest of Lung and Immune Tissue Samples in Mice

肺およびマウスにおける免疫組織サンプルの収穫との組み合わせで右室収縮期圧測定

The function of the right heart is to pump blood through the lungs, thus linking right heart physiology and pulmonary vascular physiology. Inflammation is ...

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インビトロアッセイで HIV特異的CD4 T細胞エフェクター機能に対する免疫調節経路の影響を評価する

T cell exhaustion is a major factor in failed pathogen clearance during chronic viral infections. Immunoregulatory pathways, such as PD-1 and IL-10, are ...

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Pseudomonas aeruginosa Induced Lung Injury Model

緑膿菌誘発肺傷害モデル

In order to study human acute lung injury and pneumonia, it is important to develop animal models to mimic various pathological features of this disease. ...

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Influenza infection is associated with about 36,000 deaths and more than 200,000 hospitalizations every year in the United States. The continuous ...

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

レジオネラ・ニューモ外膜小胞：マクロファージ上における炎症誘発ポテンシャルの単離と解析

Bacteria are able to secrete a variety of molecules via various secretory systems. Besides the secretion of molecules into the extracellular space or ...

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

精密カットライブ肺樹状細胞を可視化するために、マウスの肺スライスを

Inhalation of allergens and pathogens elicits multiple changes in a variety of immune cell types in the lung. Flow cytometry is a powerful technique for ...

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Many chronic airway diseases result in mucus plugging of the airways. Lungs of an individual with cystic fibrosis are an exemplary case where their ...