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DOI :
10.3791/56722-v
•
10:05 min
January 17th, 2018
Chapters
0:05
Title
1:01
Sample and Substrate Preparation
2:04
Addition of Controls and Samples
3:37
Addition of Fluorescent Conjugate and Analysis of Results
6:32
Results: Classic Disease Associated ANA and DFS70 Patterns on Conventional Hep-2 and Novel Hep-2 DFS70 Knockout Substrates
8:20
Conclusion
DFS70 自身抗体模拟常见的疾病相关的核抗体模式, 使准确的解释在使用传统的 HEp-2 基板的挑战。该协议描述了新的工程 HEp-2 基底优于传统的 HEp-2 在 ana 筛选和区分 DFS70 模式与高信心 monospecific 和混合 ana 阳性案件。
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