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DOI :
10.3791/56728-v
March 30th, 2018
Chapters
0:05
Title
0:44
Preparation of Barley Grains and Plasmid DNA for Bombardment
2:42
Particle Bombardment of Embryoless Barley Grains
4:42
Extraction of Soluble Protein from the Bombarded Grains
6:24
Measurement of GUS Activity from Grain Extracts
8:15
Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
9:50
Conclusion
향상 된 프로토콜은 입자 사격 후 보 리 aleurone 세포에서 기자 구문에서 일시적인 유전자 발현의 측정에 대 한 제공 됩니다. 96 잘 접시 효소 분석 실험 연 삭 자동화 된 곡물의 조합을 절차에 대 한 높은 처리량을 제공 합니다.
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