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二维凝胶电泳结合质谱法分析人垂体腺瘤组织蛋白质组

DOI :

10.3791/56739-v

April 2nd, 2018

April 2nd, 2018

13,042 Views

1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

在这里, 我们提出了一个二维凝胶电泳 (2DE) 与质谱 (MS) 分离和鉴定人垂体腺瘤组织蛋白质组, 这是一个良好的和可重复的2DE 模式。在使用高灵敏度 MS 分析复杂的肿瘤蛋白质组时, 每2DE 点都能观察到许多蛋白质。

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