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DOI :
10.3791/56739-v
April 2nd, 2018
Chapters
0:04
Title
1:46
Two-dimensional Gel Electrophoresis: IEF (First Dimension)
3:12
Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
7:19
Staining of 2DE-separated Proteins
8:52
Results: Separation and Identification of NFPA Tissue Proteome
10:33
Conclusion
在这里, 我们提出了一个二维凝胶电泳 (2DE) 与质谱 (MS) 分离和鉴定人垂体腺瘤组织蛋白质组, 这是一个良好的和可重复的2DE 模式。在使用高灵敏度 MS 分析复杂的肿瘤蛋白质组时, 每2DE 点都能观察到许多蛋白质。
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