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DOI :
10.3791/56742-v
January 12th, 2018
Chapters
0:05
Title
0:51
Transduction of Human Primary Keratinocytes
4:31
Cytofluorimetric Analysis of Transduced Primary Keratinocytes
6:24
Quantitative RT-PCR (qRT-PCR)
7:24
Results: Sleeping Beauty System Achieves Stable Integration of a GOI in the Human Genome
8:40
Conclusion
该协议描述了一种方法, 以实现稳定的基因的兴趣进入人类基因组由转录调控的睡美人系统。本文报道了有缺陷的慢载体的合成、人体细胞的体外转导和转细胞的分子检测。
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