Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/56745-v
•
8:55 min
December 29th, 2017
Chapters
0:05
Title
0:37
Preparation of the Microscopy Samples
1:49
Recording of the FLIM Data
3:34
Conversion of the Photon Recordings to FLIM Images
4:19
Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
6:03
Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
7:43
Conclusion
このプロトコルでは、蛍光共鳴エネルギー移動し、蛍光寿命イメージングに基づく SNARE タンパク質の複合体形成の定量的可視化を可能にする新しいメソッドについて説明します。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved