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DOI :
10.3791/56747-v
•
10:01 min
November 28th, 2017
Chapters
0:05
Title
0:51
Mass Spectrometry-based Protein Identification
4:35
Mass Spectrometry of Intact Protein Complexes (Native Mass Spectrometry)
6:17
Chemical Cross-linking Coupled with Mass Spectrometry
7:19
Results: Chemical Cross-linking and Mass Spectrometry of Multi-subunit Protein Complexes
9:14
Conclusion
蛋白质复合体的结构对其功能至关重要。结合各种质谱技术证明了研究其组装的强大功能。我们提供化学交联和本机质谱的协议, 并展示这些互补技术如何帮助阐明 multi-subunit 蛋白组件的结构。
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