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DOI :
10.3791/56747-v
•
10:01 min
November 28th, 2017
Chapters
0:05
Title
0:51
Mass Spectrometry-based Protein Identification
4:35
Mass Spectrometry of Intact Protein Complexes (Native Mass Spectrometry)
6:17
Chemical Cross-linking Coupled with Mass Spectrometry
7:19
Results: Chemical Cross-linking and Mass Spectrometry of Multi-subunit Protein Complexes
9:14
Conclusion
タンパク質複合体の建築はその機能は欠かせません。様々 な質量分析技術を組み合わせることをそのアセンブリを勉強する強力な証明しました。化学架橋とネイティブの質量分析のためのプロトコルを提供し、これらの相補的な技術が複数のサブユニット蛋白質アセンブリのアーキテクチャを明らかにする方法を示します。
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