Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/56766-v
June 12th, 2018
Chapters
0:04
Title
0:46
Production of AAV in HEK 293 Cells by Plasmid DNA Transfection
2:50
Recovery of Viral Particles, and Treatment with Serratia marcescens Endonuclease and Proteinase K
4:20
Phenol-choloroform Extraction, Ethanol Precipitation, and Preparation of Plasmid DNA Standards
5:55
Dot Blot Assay
8:46
Hybridization and Washing
10:50
Results: AAV Titration and Functional Assessment of AAP Proteins Using a Quantitative Dot Blot Assay
12:58
Conclusion
アデノ随伴ウイルス (AAV) 抗体とアセンブリ活性化の役割を研究する応用を定量化するための簡単なドット プロット法の詳細を本稿では蛋白質 (薬学会合同)、すべて AAV が見つかりましたウイルスの非構造タンパク質の新規クラス血清型、同種および異種 AAV の血清型に由来するカプシドのアセンブリを促進します。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved