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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

DOI :

10.3791/56794-v

September 19th, 2018

September 19th, 2018

6,373 Views

1Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, 751 85, Uppsala, Sweden

Capillary isoelectric focusing is an antibody-based, ultrasensitive, high throughput technique, enabling detailed characterization of proteins and their isoforms from extremely small biological samples. The following describes a protocol for detection and quantification of specific proteins and their isoforms in an automated and robotized manner.

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Keywords Capillary Isoelectric Focusing

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