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DOI :
10.3791/56801-v
•
10:06 min
February 26th, 2018
Chapters
0:04
Title
1:02
Preparing the M. tuberculosis Inoculum
3:13
Inoculating Mice with Airborne M. tuberculosis
5:33
Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
8:05
Results: Imaging
9:32
Conclusion
我们描述了使用报告酶荧光 (REF) 感染结核分枝杆菌(m 结核) 的小鼠的光学成像。该协议有助于在临床前动物模型中对m. 结核进行敏感和特定的检测, 以进行发病机制、治疗和疫苗研究。
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