Name: cavernous nerve stimulation and recording of intracavernous pressure in a rat
Uploaded: 2018-04-23T12:30:00
Description: Watch this Scientific Journal Video about Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat at JoVE.com

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根据机构指南, 动物被安置在南丹麦大学动物保育设施中。所有动物实验都是按照国家卫生研究院的指南进行的, 用于实验室动物的护理和使用。这是一个急性的, 非生存的手术程序。
1. 导管、电极及手术器械的制备
<ol><li>使用以下显微外科器械: 手术剪刀, 角度微剪刀, 纸巾钳, 一对 #7 和 #5 弯曲的微型钳, 微型针持有人, 和拉钩。 注意:由于这是一个急性的程序...

<ol>
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本研究的主要目的是描述一个简化的手术技术的小弟弟小腿插管的 ICP 记录和分离的 CN 为刺激。我们介绍了海绵体解剖的修改, 以简化手术, 并提供可重现性的记录, 增加的 ICP 与 CN 刺激。与1厘米垂直皮肤切口, 侧向基部的小弟弟, 用可触摸的坐骨股骨作为指导, 我们取得了良好的暴露 ischiocavernosus 肌肉和膜。此过程比文献中描述的更快 (低于15分钟), 导致最小组织中断2,<sup...

体内对实验动物勃起功能的研究始于 1863年, 埃克哈德1的开创性实验工作。刺激的骨盆神经被用来诱发更高的 ICP 的狗。在整个 20th世纪, 类似的实验性协议被用于大型动物, 如狗, 猴子, 猫和兔子。在 1989年2中, 由昆兰et 等) 首次开发出大鼠勃起功能的评价。此后, 该方法已被其他几个组34修改和更新。今天, 大鼠是最广泛使用的动物模型, 研究勃起功能障碍的病理学和评估新的治疗方案。该程序的主要步骤包括, 记录全身血压使用颈动脉线, 插管的小弟弟小腿, 以测量 icp, 并刺激 CN 诱导增加的 icp。虽然有几个研究人员对模型进行了改进, 但其重现性仍然是一个问题, 不同的实验室也报告了可变的结果。有几个陷阱仍然存在。
上一篇文章5,6,7,8,9,10描述使用全小弟弟暴露与脱的生殖器海绵体插管。这不是一个最佳的方法, 因为操纵和破坏性解剖导致结构损伤, 这是必不可少的勃起功能。CN 的解剖已被很好地描述为10,11, 但是由于多种因素可能会影响实验结果, 神经的刺激不是最佳的。CN 刺激技术包括通过拉住神经周围的双极钩电极来解除周围组织的神经, 并在每次刺激前干燥神经。这可能导致各种程度的神经损伤和电流泄漏, 从而导致通过刺激替代途径例如、盆底肌肉、膀胱和胃肠道而减少反应或假增加 ICP道12。所有这些因素都限制了重现性。
在我们的研究中, 我们观察到, 麻醉的深度和类型都对 ICP 产生了深远的影响。使用的麻醉剂是戊巴比妥钠, 氯胺酮/甲苯噻嗪或氯胺酮/咪达唑仑注射液, 或异氟醚/氧吸入。
在这里, 我们描述了一个简化的手术方法, 并提供数据支持标准化的实验协议。

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cavernous nerve stimulation and recording of intracavernous pressure in a rat

海绵状神经 (CN) 的刺激和海绵压力的测量已被广泛用于测试和评估勃起功能障碍的治疗方法。然而, 所使用的方法在实验室之间有所不同, 而且仍然存在陷阱。这项研究的目的是描述一种外科技术, 将提供一个可靠的和可重复的模式。通过暴露 ischiocavernosus 肌肉在其插入点上的坐骨股骨, 小弟弟小腿可能是空心的最小解剖和伤害的结构涉及勃起功能。在不需要提升和干燥的情况下, 对 CN 进行反复刺激, 通过使用125µm 双极银电极和生物相容的硅胶来分离电极-神经复合体。该方法通过减少伸展和干燥神经, 提供完全的神经隔离, 消除电漏, 防止刺激的替代途径, 防止 neuropraxia。

使用本协议的建议刺激设置, 在吸入麻醉下, 异氟醚2.0% 氧0.8 升/分, 应产生的结果, 如图 11和图12, 其中有多个回到背面刺激之间的75-80 毫米汞.图 13显示与20分钟的激发相同的稳定响应, 其响应稳定在73-77 毫米汞. 通过冲洗管道和敲击小腿 (图 9) 来测试 ICP 测量的线路。回复基线的快?...

Watch this Scientific Journal Video about Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat at JoVE.com

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

本研究介绍了用硅胶胶和海绵压力测量分离神经电极复合物进行海绵状神经刺激的简化手术方法和技术。

大鼠海绵状神经刺激与海绵压力记录

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for ...

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Renal sympathetic nerves contribute significantly to both physiological and pathophysiological phenomena. Evaluating renal sympathetic nerve activity ...

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Mutations in the CCM1 (aka KRIT1), CCM2, or CCM3 (aka PDCD10) gene cause cerebral cavernous malformation (CCM) in humans. Mouse models of CCM disease have ...

Vagus Nerve Stimulation As an Adjunctive Neurostimulation Tool in Treatment-resistant Depression

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Vagus nerve stimulation (VNS) is an approved neurostimulation therapy. The purpose of the method is to treat patients with therapy-resistant depression ...

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