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DOI :
10.3791/56846-v
March 7th, 2018
Chapters
0:04
Title
1:01
Affinity Purification of FLAG-tagged hEAG1 Channel Protein from HEK-293 Cells
4:18
BCA Assay, Western Blotting, and Labeling the Purified Protein with Biotin for the BLI Assay
6:10
Bio-layer Interferometry Assay
8:06
Results: Interaction Kinetics of hEAG1 with PIP2 Using the Bio-layer Interferometry Assay
9:35
Conclusion
ここのプロトコルでは、小分子脂質リガンド ホスファチジルイノシトール 4, 5-ビスリン酸 (PIP2) と精製 hEAG1 イオン チャネル蛋白質の相互作用について説明します。測定は、バリでした小分子イオン チャネル リガンドのスクリーニングのための潜在的な方法であることを示します。
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