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DOI :
10.3791/56855-v
August 22nd, 2018
Chapters
0:04
Title
0:48
Sample Preparation
4:20
Electrophoresis, Transfer, and Block
5:30
Antibody Labeling and Detection
6:09
Results: Use of Optimized Western Blotting Technique to Analyze Small Numbers of Hematopoietic Stem and Progenitor Cells
7:56
Conclusion
しみが付くプロトコル標準ウエスタンとして 500 造血幹前駆細胞の数を分析するため最適化を行った。最適化を含むセルのサンプルの処理、管の転送を制限して塩化物イオンとサンプル バッファー内セルを直接溶解に注意してください。
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