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DOI :
10.3791/56885-v
•
8:10 min
August 21st, 2018
Chapters
0:04
Title
1:19
Sample Preparation
2:22
Defining the Linear Range of the QDB Analysis
3:19
Process of QDB Analysis
6:11
Results: Representative Result of a QDB Analysis to Determine the Absolute CAPG levels in Three Mouse Tissues
6:56
Conclusion
在这里, 我们展示了定量斑点杂交分析 (QDB) 的详细过程, 通过确定目标蛋白的绝对含量, 帽肌动蛋白, gelsolin 样 (CAPG), 在三种不同的小鼠组织。我们展示了高通量, 方便, 定量应用免疫印迹技术的生物标志物验证在细胞和组织水平。
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