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DOI :
10.3791/56889-v
•
7:53 min
January 1st, 2018
Chapters
0:05
Title
1:15
Preparation of RNAi Plates
2:37
Preparation of C. elegans Strain BAT28
4:56
Induction of Germ Cell Reprogramming
6:01
Results: Assessing Reprogrammed Cells
6:52
Conclusion
本协议描述了如何在细胞命运转换期间研究在线虫线虫体内的细胞过程。利用转基因动物, 允许热休克启动子驱动的神经元的过度表达, 诱导转录因子 CHE-1 和 rna 介导的染色质调节因子 LIN-53 生殖细胞对神经元重新编程的损耗可以观察在体内。
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