Name: evaluation of blood lactate and plasma insulin during high-intensity exercise by antecubital vein catheterization
Uploaded: 2018-05-18T12:30:00
Description: Watch this Scientific Journal Video about Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization at JoVE.com

作者希望感谢 p. Karagiannis 博士阅读论文和好友教授的技术支持。

这里描述的所有方法都得到了当地道德委员会 (同志社大学伦理委员会: 15033) 的批准, 严格按照《赫尔辛基宣言》规定的标准。所有与会者都获得了知情同意。参加者为男性休闲运动员 (年龄, 20.1 岁, 1.2 岁; 体重, 69.7 英寸6.2 公斤; 身高, 176.1 厘米5.8 公分; 身体脂肪 13.6 @ 1.5% 和 VO2最大, 52.6 @ 6.6 毫升/分钟1/千克1)。
1. 制定《行动议定书》
<p class="jove_conten...

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在本研究中, antecubital 静脉插管, 在高间隔运动中成功采集血液标本。以此方法为例, 对血浆胰岛素的血乳酸和血浆分离后的血液标本进行了分析。这种方法的主要优点是它允许在短时间内采集血样。同时, 它也有一些局限性。例如, 允许使用的有限的锻炼方式;需要的助理人数, 以及所需的专门培训人员;和低样本量的风险, 由于在前臂的低血压。
对于一个成功的血样采集, 该主?...

身体活动过程中代谢和内分泌标志物的测量与理解不同运动方式的生理影响有关。在某些锻炼方式 (例如高强度间隔练习 (HIIE)) 中, 血液代谢物和荷尔蒙水平在相对较短的时间内波动1,2,3。
许多体育运动 (即,足球、篮球和橄榄球) 由低强度运动和/或被动期间与4、5之间的短时间高强度运动组成。HIIE 与上述运动相似, 因此被广泛用作运动员的训练方法6。此外, 由于其与运动的相似性, HIIE 已被用作研究运动生理学的模型7。采血是衡量生理变量的关键, 它经常被用于研究目的和身体状况的评估。然而, HIIE 期间的血液取样在技术上是困难的, 因为样品收集之间的时间很短。为解决这一问题, 我们在这里提出了一种方法来获得血液样本的 antecubital 静脉插管在短时间内, 而受试者执行 HIIE。

<table border="0" cellpadding="0" cellspacing="0" width="626">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Cycle ergometer</td>
			<td style="width:71px;">Monark</td>
			<td colspan="2" style="width:339px;">MONARK Ergomedic 874 E</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Catheter</td>
			<td style="width:71px;">Terumo</td>
			<td style="width:172px;">SR-FF2032</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Vacutainer</td>
			<td style="width:71px;">Terumo</td>
			<td>PZ-D03</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Tube for catheter</td>
			<td style="width:71px;">Terumo</td>
			<td style="width:172px;">SP-PTW30L02</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Heparin</td>
			<td style="width:71px;">Terumo</td>
			<td>PF-10HF10UA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lactate pro2</td>
			<td style="width:71px;">Arkray</td>
			<td>&#65324;&#65332;-1730</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Insulin ELISA kit</td>
			<td>Abnova</td>
			<td>ELISA Kit KA0921</td>
			<td></td>
		</tr>
	</tbody>
</table>

evaluation of blood lactate and plasma insulin during high-intensity exercise by antecubital vein catheterization

身体活动过程中代谢和内分泌标志物的测量与理解不同运动方式的生理影响有关。在一些锻炼方式 (例如,高强度间隔练习) 中, 血液代谢物和荷尔蒙水平在短时间内会发生变化。在本研究中, 我们描述了一种 catheterize antecubital 静脉的方法, 它允许在运动过程中采集几个血样。应用该方法对高强度运动中的胰岛素和静脉乳酸浓度进行测定。演习包括三三十年代的高强度运动, 由4分钟的恢复分离。在上一个恢复期之后, 执行了一个盖特测试。antecubital 静脉的血液样本是在三十年代前后, 以及在盖特试验前后获得的。因此, 在运动过程中, 可以对血浆胰岛素和静脉血乳酸的变化进行评价。

作为有代表性的结果, 我们使用该协议来测量在一个周期测力计 HIIE 中的六个实验对象的血乳酸和胰岛素的变化。盖特试验的峰值功率和平均功率分别为 732 120 和549瓦84瓦特。在三十年代 HIIE 期间, 平均功率用于计算功率输出 (494 瓦75瓦特)。
图 2显示了 HIIE 期间血浆胰岛素和血乳酸的变化。血浆胰岛素 (<str...

Watch this Scientific Journal Video about Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization at JoVE.com

Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization

在这里, 我们提出了一个协议, 以获得几个血液样本从 antecubital 静脉在高强度间歇训练。该协议可能有助于测量血液代谢物和内分泌标记在运动期间。

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