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DOI :
10.3791/56892-v
•
8:49 min
March 20th, 2018
Chapters
0:04
Title
0:30
Nematode Growth Medium (NGM) Plates and C. elegans Mass Culture Preparation
3:24
Hatch in Buffer to Obtain Synchronized Culture of Arrested Larva
5:01
Setup and Chemical Treatment of 96-well Culture/Assay Plates
6:42
Results: Representative Compound Screening and Re-testing
8:11
Conclusion
在这里, 我们描述了一个简单的协议, 快速生产数以百计的线虫生长培养基琼脂, 96 良好的文化板块, 其数量一致的Caenorhhabditis 线虫每井。这些文化对于整个有机体的表型筛选是有用的。我们专注于利用这些文化来筛选化学物质以促进长寿效果。
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