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DOI :
10.3791/56892-v
•
8:49 min
March 20th, 2018
Chapters
0:04
Title
0:30
Nematode Growth Medium (NGM) Plates and C. elegans Mass Culture Preparation
3:24
Hatch in Buffer to Obtain Synchronized Culture of Arrested Larva
5:01
Setup and Chemical Treatment of 96-well Culture/Assay Plates
6:42
Results: Representative Compound Screening and Re-testing
8:11
Conclusion
ここで急速に線虫成長メディア寒天、ウェルあたりCaenorhhabditis 線虫の一貫性のある数 96 ウェル培養皿の数百を生成するための単純なプロトコルについて述べる。これらの文化は、全有機体の表現型スクリーニングに適しています。ここでプロの長寿効果のスクリーン化学物質にこれらのカルチャを使用して注力します。
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