Name: effectiveness of the air stripping in two salmonid fish, rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta morpha fario)
Uploaded: 2018-09-16T13:00:00
Description: Watch this Scientific Journal Video about Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout Oncorhynchus Mykiss and Brown Trout Salmo Trutta Morpha fario at JoVE.com

提出的研究得到了 "鱼剥的气动方法-可能的应用, 对配子的质量和数量和鱼的福利的影响" (缩写: PNEUFISH) 资助下的 "渔业业务计划的发展2007-2013 区和沿海地区 "(OR-61724-OR1400001/10), 拨给动物繁殖和食品研究研究所的资金波兰科学院, 波兰奥尔什丁, 成本办公室 (粮食和农业费用行动 FA1205: AQUAGAMETE).我们要感谢, 斯坦利 Ifeanyi Ugwu 在英语编辑方面的大力支持。我们也要承认动画制片人 "工作室 Filmowe Ruchome Obrazki"。

程序是根据波兰奥尔什丁动物实验伦理学地方委员会进行的。
1.Equipment. 准备
<ol><li>用注射器和针头将气体注入鱼体内腔。</li>
	<li>设置气体压力在0.5 酒吧, 并保持这个压力在1酒吧在整个。</li>
	<li>控制气流。对于鲑鱼鱼来说, 1.5 升/分是最有效的气体耗尽速度。保持最佳速度, 因为较高的速度可能不会得到所有的鸡蛋, 而速度慢可能不必要地延长的过程。</li>
	<li>将注射器与?...

<ol>
	<li>Nelson, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Fishes of the World. , (2006).</li><li>Garc&#237;a-Mar&#237;n, J. L., Sanz, N., Pla, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proportions+of+native+and+introduced+brown+trout+in+adjacent+fished+and+unfished+Spanish+rivers.">Proportions of native and introduced brown trout in adjacent fished and unfished Spanish rivers.</a> Conservation Biology. 12, 313-319 (1998).</li><li>Dietrich, G. J., Wojtczak, M., Slowinska, M., Dobosz, S., Kuzminski, H., Ciereszko, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Broken+eggs+decrease+pH+of+rainbow+trout+(Oncorhynchus+mykiss)+ovarian+fluid.">Broken eggs decrease pH of rainbow trout (Oncorhynchus mykiss) ovarian fluid.</a> Aquaculture. 273, 748-751 (2007).</li><li>Zurbuch, P. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+structure+for+easy+fish+recovery+during+drainage+of+an+impoundment.">A structure for easy fish recovery during drainage of an impoundment.</a> Progressive FishCulturist. 27, 237-238 (1965).</li><li>Cejko, B. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+different+stripping+methods+of+female+and+activation+medium+on+fertilization+success+in+northern+pike+(Esox+lucius).">Effects of different stripping methods of female and activation medium on fertilization success in northern pike (Esox lucius).</a> Czech Journal of Animal Sciences. 10, 481-486 (2016).</li><li>Morisawa, S., Morisawa, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+potential+for+sperm+motility+by+bicarbonate+and+pH+in+rainbow+trout+and+chum+salmon.">Induction of potential for sperm motility by bicarbonate and pH in rainbow trout and chum salmon.</a> Journal of Experimental Biology. 136, 13-22 (1988).</li><li>Billard, R., Cosson, J., Perchec, G., Linhart, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biology+of+sperm+and+artificial+reproduction+in+carp.">Biology of sperm and artificial reproduction in carp.</a> Aquaculture. 129, 95-112 (1995).</li><li>Iuchi, I., Ha, C. R., Sugiyama, H., Nomura, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+chorion+hardening+of+eggs+of+rainbow+trout,+Oncorhynchus+mykiss.">Analysis of chorion hardening of eggs of rainbow trout, Oncorhynchus mykiss.</a> Development Growth and Differentiation. 38, 299-306 (1996).</li><li>Wojtczak, M. A., Kowalski, R. K., Dobosz, S., Goryczko, K., Ku&#378;minski, H., Glogowski, J., Ciereszko, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+water+turbidity+for+evaluation+of+rainbow+trout+(Oncorhynchus+mykiss)+egg+quality.">Assessment of water turbidity for evaluation of rainbow trout (Oncorhynchus mykiss) egg quality.</a> Aquaculture. 242, 617-624 (2004).</li><li>Tabrizi, E. N., Khara, H., Nezami, S. A., Lorestani, R., Shamspour, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Broken+Eggs+Influence+on+Fertilization+Capacity+and+Viability+of+Eggs,+Turbidity+and+pH+of+Ovarian+Fluid+and+Fertilization+Water+in+the+Endangered+Caspian+Brown+Trout,+Salmo+Trutta+Caspius.">Broken Eggs Influence on Fertilization Capacity and Viability of Eggs, Turbidity and pH of Ovarian Fluid and Fertilization Water in the Endangered Caspian Brown Trout, Salmo Trutta Caspius.</a> International Journal of Biology. 3 (1), 161-166 (2011).</li><li>Carl, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beware+of+the+broken+egg!+A+possible+cause+of+heavy+losses+of+salmon+eggs.">Beware of the broken egg! A possible cause of heavy losses of salmon eggs.</a> Progresive Fish-Culturist. 53, 30-31 (1941).</li></ol>

从鱼中采集卵子的气动方法, 虽然比传统的方法 (手剥) 更耗时, 但能保证成熟卵母细胞的高质量 (这项工作)。这与在应用这个过程中的鸡蛋机械破坏的风险低有关。卵巢液的高 pH 值, 以及施肥率的提高, 证明了气动方法的实用性。总的来说, 在孵化协议的标准化方面, 在鱼类繁殖中收集卵子的空气剥离方法可能是一个很有希望的选择, 因为它们的成功应用与操作者的手工优异度无关。
<p class="jove_c...

虹鳟 (虹鳟鱼虹鳟鱼) 和褐鳟鱼 (鲑鲑形态石斑鱼) 属于鲑1的 Salmoniformes 家庭。由于商业和娱乐的重要性, 这两个物种在全球水产养殖中的产量得到了快速增长。在波兰, 鲑鱼鱼的产量约为 20 000 吨, 彩虹鳟鱼是主要物种。另一方面, 褐色鳟鱼是欧洲淡水鱼类资源的主要来源, 因为它在水产养殖中具有商业价值, 对钓鱼的重要性。在许多水生生态系统中, 褐鳟鱼种群的存在受到威胁。因此, 人工繁殖已被应用于重新放养本种2的当地人口。
彩虹鳟鱼和褐色鳟鱼通常在三岁时达到性成熟, 男性比女性早一年成熟1。在人工条件下, 彩虹鳟鱼和褐色鳟鱼的卵和精子通常由温和的腹部按摩收集。在实践中, 在小规模上, 采用手剥法采集鸡蛋是具有成本效益的。然而, 在很大程度上, 手剥法可能是劳动密集型和精疲力尽。因此, 由于孵化工人的厌学, 这种技术可能会导致鸡蛋破碎。卵的破损经常导致卵巢液 pH 值降低, 蛋黄释放, 这两种情况对卵3的受精有负面影响。此外, 这种传统的卵子收集方法 (反复按压腹部) 也可能导致因皮肤感染而引起的亲鱼死亡, 与粘液屏障的恶化有关。
在 1957年, 澳大利亚首次使用气动方法收集鸡蛋.4。该方法的基础是注入气体 (空气, 氮气或氧气) 进入体腔, 以驱逐鸡蛋使用气体压力。这种快速和简便的技术已成功地应用于鲑鱼鱼, 没有任何负面的副作用对鱼4。最近, 这种方法用于野生物种 (北派克,狗鱼卢修斯) 收集成熟的鸡蛋在人工条件5。结果表明, 除受精 (Woynarovich 溶液、Billard 缓冲液或孵化水)5, 与手工剥蛋相比, 空剥蛋具有更高的受精和孵化率。
本研究的主要目的是与传统的手剥法相比, 对彩虹鳟鱼和褐鳟中采集鸡蛋的气动方法 (空气剥离) 进行试验。比较了两种方法的效率 (获得的卵量和采集时间、配子质量 (卵巢液 pH 值) 以及孵化率和产卵后死亡率)。

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			<td height="21" style="height:21px;width:216px;">Glycine pure P.A.</td>
			<td style="width:215px;">AVANTOR</td>
			<td align="right" style="width:119px;">527560117</td>
			<td style="width:167px;">sperm activating buffer</td>
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			<td height="21" style="height:21px;width:216px;">Trizma base</td>
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			<td height="21" style="height:21px;width:216px;">Sodium chloride Bioreagent</td>
			<td style="width:215px;">Sigma Aldrich</td>
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			<td>sperm activating buffer</td>
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			<td height="21" style="height:21px;width:216px;">Calcium chloride anhydrous</td>
			<td style="width:215px;">Sigma Aldrich</td>
			<td style="width:119px;">C4901</td>
			<td>sperm activating buffer</td>
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			<td height="42" style="height:42px;width:216px;">Propiscin (0.2% etomidate solution)</td>
			<td style="width:215px;">IRS Olsztyn, Poland</td>
			<td style="width:119px;">not indicated</td>
			<td></td>
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		<tr height="42">
			<td height="42" style="height:42px;width:216px;">compressor</td>
			<td style="width:215px;">Thomas&#160; Sheboigan WI USA&#160;</td>
			<td style="width:119px;">DT/SR 070800001882</td>
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			<td height="21" style="height:21px;width:216px;">reduction valve</td>
			<td style="width:215px;">Camozzi&#160;</td>
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			<td height="21" style="height:21px;width:216px;">air hose</td>
			<td style="width:215px;">ZEC T.P.U.</td>
			<td style="width:119px;">&#160;SH.98&#160;</td>
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			<td height="21" style="height:21px;width:216px;">syrgine</td>
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			<td height="21" style="height:21px;width:216px;">Air spawning stage</td>
			<td style="width:215px;">Biopasz, Poland</td>
			<td style="width:119px;">PNEU001</td>
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			<td height="42" style="height:42px;width:216px;">Orion Ross Ultra electrode</td>
			<td style="width:215px;">Thermo Scientific, Waltham, MA, USA</td>
			<td style="width:119px;">8102BNUWP</td>
			<td></td>
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effectiveness of the air stripping in two salmonid fish, rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta morpha fario)

卵收集是鲑鱼孵化场鱼类繁殖过程中最关键的过程之一。经典的方法包括使用手按摩的鱼腹部, 以驱逐鸡蛋。另一种方法是使用注入体内腔的气体压力, 从而导致卵的随后释放。认为这种方法对亲鱼的福利和蛋质有较小的负面影响。本文比较了两种鲑鱼鱼、虹鳟 (虹鳟鱼虹鳟鱼) 和褐鳟 (鲑鲑形态石斑鱼) 的一年生存期、卵量和品质的不同的空气和手剥离方法的结果。结果表明, 在彩虹鳟鱼中, 空气剥离使卵质量更好, 一年存活率更高。此外, 空气剥离导致的死亡率低于手剥离组 (25% vs35%)。手剥组的 pH 和孵化率低于剥离组。在褐鳟鱼的情况下, 用手和气剥法获得的卵质量相似;然而, 在空气剥离组中, 鱼的一年损失更高 (15% 与0% 的手剥鱼相比)。尽管空气剥离法在鸡蛋质量方面的优势可能在所有鲑鱼物种中都看不到, 但在孵化场中, 空气剥离过程可能是一个很有希望的选择, 因为它确保了高水平的重现性。和效率。

从鲑鱼鱼孵化场 (Rutki-Żukowo, 波兰奥尔什丁内陆渔业研究所) 获得了彩虹鳟鱼 (年龄 2 + 1700 328 克) 和褐色鳟鱼 (年龄 3 +, 1900 @ 435 克) 的成熟雄性和雌性。男性和女性被安置在分开的坦克与容量大约 5 m3。水温12±1°c。在操作之前, 鱼被麻醉了以 0.2% Propiscin。鱼被分成两组。他们中的一部分接受了一种传统的方法来获得卵子,即腹部压力 (n = 20)。剩余的?...

Watch this Scientific Journal Video about Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout Oncorhynchus Mykiss and Brown Trout Salmo Trutta Morpha fario at JoVE.com

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout Oncorhynchus Mykiss and Brown Trout Salmo Trutta Morpha fario

本研究的主要目的是规范和测试在彩虹鳟鱼和褐鳟鱼中采集鸡蛋的气动方法 (空气剥离)。这种方法可以有效和简单的收集鸡蛋没有鱼腹部按摩的必要性。

两鲑鱼鱼、虹鳟 (虹鳟鱼虹鳟鱼) 和褐鳟 (鲑鲑形态石斑鱼) 的空气剥离效果

Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on ...

鱼蛋的空气剥离，程序的有效性，以及对两种鲑鱼，虹鱼和棕鱼的育雏种群管理的影响。嗨，我叫拉多斯瓦夫·科瓦尔斯基我们来自奥尔斯提恩的波兰科学院动物繁殖和食物研究所。我们想介绍鲑鱼的空气产卵方法。手工剥落是鱼类的传统产卵程序。此过程可能会用手对卵子造成损害，从而降低卵子的受精电位。另一种方法利用气体压力从鱼体内消耗鸡蛋。此方法称为空气生成。空气产卵是一种在不按摩鱼腹部的情况下收集鱼卵的方法。注射器和针头用于将气体注射到鱼体腔中。鱼蛋的空气剥离。在此过程中，应控制气体压力，并在其值低于一个柱线时保持最佳状态。还应控制气流。对于三文鱼来说，每分钟1升半是气体耗尽的高效速度。应保持最佳速度，因为较高的速度可能不会获得所有的鸡蛋，而较慢的速度可能会不必要地延长程序。注射器应与拧紧的针头连接，以避免气体压力导致针头分离。鱼应该麻醉，在产卵前小心地干涸。鱼被放置在倾斜的床上约35至40度。针应迅速插入腹部鳍下方。鸡蛋被气体自由去除，直到只有奇异的鸡蛋从鱼体内取出。腹部的温和按摩有助于去除鱼体内多余的气体。产卵后，鱼被轻轻地转移到水箱与水流。空气产卵后释放到水箱中的鱼最初可能在水面上游来游去。几分钟后，鱼在游泳时释放剩余的气体，开始正常游泳。获得的鸡蛋非常干净，没有血液或粪便污染。结果。通过手工剥离方法采集卵子的持续时间短于两种物种的空气剥离。剥离方法并不影响手工或空气剥离方法获得的卵子总量。手部剥离后虹鱼的卵巢液低于空气剥离后。空气剥离后，虹鱼的孵化率也显著提高。结论，空气剥离不快于手剥离方法。空气剥离有利于彩虹鱼蛋的质量，但对棕色的鱼蛋没有好处。由于进行空气剥离程序不需要特殊技能，熟练和非熟练的孵化工人都可以获得类似的卵子数量和质量。空气剥离程序是新孵化场寻求鸡蛋收集程序的一个有希望的选择，其特点是高可重复性和效率，以及很少或没有经验的运营商。

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Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫

Studying the cell biological processes during converting the identities of specific cell types provides important insights into mechanism that maintain ...

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

鸟类模型中器官形成早期和晚期的两步探讨: 胸腺和甲状旁腺器官发生范式

The avian embryo, as an experimental model, has been of utmost importance for seminal discoveries in developmental biology. Among several approaches, the ...