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DOI :
10.3791/56904-v
•
9:07 min
February 5th, 2018
Chapters
0:04
Title
1:02
Preparation of Skin Fibroblasts for Reprogramming
1:43
Plating for Reprogramming (Day 1)
3:22
Viral Transduction (Day 0)
4:12
Maintenance of the Converting Cells
4:58
FACS
7:33
Results: Generation of Induced Neurons from Adult Human Dermal Fibroblasts
8:15
Conclusion
直接的神经元重编程产生的神经元保持起始体细胞的年龄。在这里, 我们描述了一个单一的矢量的方法, 产生诱导神经元从真皮成纤维细胞获得从成人捐赠人。
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