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DOI :
10.3791/56915-v
December 12th, 2017
Chapters
0:05
Title
0:52
Transfecting of HEK-293T Cells Using Calcium Phosphate
2:19
Harvesting Virus
2:50
Concentration of Viral Particles by Ultracentrifugation
5:11
Esimating of Viral Titers Using p24-enzyme-linked Immunosorbent Assay
7:31
Counting GFP-positive Cells
8:25
Results: Production and Validation of the Knockout-efficiency of Integrase-deficient Lentiviral-CRSPER/Cas9 Vector
9:57
Conclusion
セルに CRISPR/Cas9 を提供するための車としてインテグラーゼ欠損レンチウイルスベクター (IDLVs) の生産戦略について述べる。迅速かつ堅牢な遺伝子がセル内編集を仲介する能力、IDLVs 現在安全と同様に効果的なベクトル遺伝子送達用に比べてベクトル インテグラーゼ主務。
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