Culture Preparation of Dissociated Adult DRG Neuron
3:41
Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
5:47
Neurite Outgrowth Assay with Collected Conditioned Medium
7:05
Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
7:55
Conclusion
Transcript
The overall goal of this procedure is to generate macrophages that can secrete molecular factors promoting neurite growth. This method can help answer key questions on how neurons and macrophages interact with each other to activate the pro-regene
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The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.