Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth ActivityHyeok Jun Yun 1,2, Eun-Hye Kim 1,2, Byung Gon Kim 1,2,3
1Department of Brain Science, Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University School of Medicine, 3Department of Neurology, Ajou University School of Medicine
The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.
