Name: visual evoked potential recordings in mice using a dry non-invasive multi-channel scalp eeg sensor
Uploaded: 2018-01-12T15:00:00
Description: Watch this Scientific Journal Video about Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor at JoVE.com

这项工作得到了部分的支持, 重点研究机构, 重点-加州理工研究合作项目通过一笔赠款提供的主旨在2017年。也得到由韩国政府 (MEST) 资助的国家研究基金会 (NRF) 的研究补助金 (NRF-2016R1A2B4015381) 和由科学、信息和通信技术部资助的韩国脑研究所的 KBRI 基础研究方案和未来计划 (17-BR-04)。

动物照料和处理遵循了光州科学和技术研究所 (要点) 的体制方针。
注: 从鼠标中获取视觉诱发电位信号的过程包括四步骤: (1) 传感器组件, (2) 动物制剂, (3) 诱发电位测量, (4) 信号处理。
1. 传感器总成
<ol><li>为一个非侵入电极准备十六针。 注: 每个针型电极由三部分组成: 探头头柱塞、内部弹簧和枪管, 如图 1a所示。每个引...

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我们首先关注的是传感器的设计, 通过最小化复杂的手术程序来确定实用性。可变形 EEG 传感器由十六针组成: 十四用于记录, 一个用于地面, 最后一个用于参考电极。每个电极都有柱塞弹簧筒结构, 它将变形能力应用到电极的接触面上, 从而便于从弯曲的小鼠头皮上获得均匀稳定的信号。考虑到动物的福祉, 我们试图通过扩大皮肤电极界面的接触面积29来减轻弹簧力所引起的疼痛, ?...

随着老年痴呆症、老年痴呆症、帕金森综合征和中风的患者数量的增加, 随着人口老龄化和预期寿命的增加, 这些疾病的长期社会负担已还增加了1、2、3。此外, 大多数神经发育疾病, 如精神分裂症和自闭症, 伴有认知和行为障碍, 影响患者的整个生活2,3,4。因此, 研究人员一直在努力改善诊断, 预防, 病理理解, 长期观察和治疗脑部疾病。然而, 问题仍然源于大脑的复杂性和未疾病的病态。翻译研究可能是一个有前途的工具, 以确定解决方案, 因为它使基础研究的转移, 以更短的时间框架内的临床应用, 以较低的成本, 并与更高的成功率在神经科学领域5 ,6,7。翻译研究的另一个目标是检查人体的适用性, 这需要在动物身上进行非侵入性的实验方法, 以便与人类的相同方法进行比较。这些条件已经导致了一些重要的需要发展非侵入性动物的准备方法。一种方法是脑电图 (eeg), 它揭示了皮质脑连接和活动 two-dimensionally 的高时间分辨率, 并受益于非侵入性的协议。事件相关电位记录 (ERP) 是利用 EEG 的典型实验范式之一。
以往的许多研究都采用了非侵入性脑电图方法, 针对人类的对象, 而侵入的方法, 如植入螺钉和极型电极, 已被用于动物研究8,9,10,11,12. 这些方法的信号质量和特性明显依赖于传感器位置的侵入性。为成功的翻译研究, 加纳强调使用相同的条件进行动物研究的那些用于人类研究13。然而, 对于使用动物的基础研究, 非侵入性脑电图方法并不普遍。一种新的方法, 使用非侵入性头皮脑电图传感器系统的重点是实验室小鼠将是一个可靠的和有效的工具, 可用于人类的非侵入性的范式的转化研究, 以及。
众多的鼠标脑电图研究 led 的方式商业化 PCB (印制电路板) 基于多通道电极14,15,16。虽然他们采取了侵入性的方法, 他们有一个有限的通道数 (3-8), 这使得它更难观察 large-scale 脑动力学。此外, 其侵入和高成本也可以限制应用。在另一项研究中, KIST (韩国科学和技术研究所) 开发了40通道 polyimide-based 薄膜电极, 并将其连接到鼠标的头骨17,18,19,20.这项工作获得了最大数量的鼠标脑电图通道。然而, 它是机械薄弱, 不容易重用;因此, 它不适合长期观察, 导致信号减弱, 可能是由免疫反应引起的。同时, Troncoso 和 Mégevand 获得了一种感官诱发电位 (SEP) 对啮齿动物的头骨与三十二不锈钢电极的穿孔聚 (甲基丙烯酸甲酯) (PMMA, 丙烯酸玻璃) 网格21,22,23. 尽管信号质量高, 但电极具有机械弹性和柔软性;因此, 他们在多项实验中遇到了困难。此外, 这种方法仍然是微创的。虽然这些方法提供了良好的信号质量, 但小鼠颅骨的表面积有限, 因此电极的数量受到限制, 使用的是不锈钢极型电极。以前的一些小鼠脑电图研究显示了一些局限性。在本研究中, 我们将展示一种新的测量脑电图的方法, 适用于临床前的翻译研究使用无创干式多通道传感器。
为了克服以往动物脑电图方法的局限性, 包括动物制剂的内在复杂性、侵袭性、高成本、浪费和弱机械强度, 我们试图开发一种新的电极, 它展示了灵活性、干式状态、多通道功能、侵入和性。在下面的协议, 我们将描述的过程, 测量视觉诱发电位 (诱发电位) 记录鼠标头皮使用干燥, 无创, 多通道脑电图传感器。这种方法利用了很容易获得的资源, 从而降低了进入生物医学工程领域的动物实验的门槛。

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visual evoked potential recordings in mice using a dry non-invasive multi-channel scalp eeg sensor

对于实验室小鼠的头皮脑电图研究环境, 我们设计了一种干式16通道脑电图传感器, 由于柱塞弹簧筒结构面和金属的机械强度, 它是无创、可变形和可重用的。材料.从小鼠体内获取视觉诱发电位反应的整个过程包括四步骤: (1) 传感器组件, (2) 动物制剂, (3) 诱发电位测量, (4) 信号处理。本文介绍了具有微米电压信号分辨率和 sub-hundred 毫秒时间分辨率的多小鼠诱发电位反应的有代表性的测量方法。虽然所提出的方法比其他以前报道的动物脑电图获取方法更安全、更方便, 但仍存在一些问题, 包括如何提高信噪比以及如何将这种技术应用于自由运动的动物。该方法利用了较易获得的资源, 并以满意的信号质量显示重复的诱发电位响应。因此, 该方法可用于纵向实验研究和利用非侵入范式进行可靠的平移研究。

我们计算了十一只小鼠诱发电位反应的平均集合, 如图 2所示。这一结果表明, 通过这个实验获得的诱发电位反应从预期 (-300 毫秒) 到 post-stimulation 周期 (600 毫秒), 因为刺激是在时间 0 s。值得注意的是, 信号在刺激后仅波动一段时间 (少于300毫秒), 而信号在 post-stimulation 期间稳定稳定。此外, 十四通道可以分为几个组的基础上, 视觉诱发电位反应, 揭示?...

Watch this Scientific Journal Video about Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor at JoVE.com

Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor

我们设计了一种干式16通道脑电图传感器, 它是无创、可变形和可重用的。本文介绍了利用干无创多通道脑电传感器对小鼠头皮上的视觉诱发电位 (诱发电位) 信号进行处理的全过程。

干无创多通道头皮脑电传感器在小鼠视觉诱发电位记录中的应用

For scalp EEG research environments with laboratory mice, we designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable ...

Research

JoVE Journal

Neuroscience

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

电极的制作及植入海兔californica完整的，自由的行为的动物多通道的神经和肌肉的录音

Recording from key nerves and muscles of Aplysia during feeding behavior allows us to study the patterns of neural control in an intact animal.  ...

科研

神经科学

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a ...

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

transretinal ERG的录音，从小鼠视网膜杆和锥光反应

There are two distinct classes of image-forming photoreceptors in the vertebrate retina: rods and cones. Rods are able to detect single photons of light ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

双突触诱发的星形胶质细胞和神经元的反应，在急性海马脑片电生理记录

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

Extracting Visual Evoked Potentials from EEG Data Recorded During fMRI-guided Transcranial Magnetic Stimulation

提取脑电数据视觉诱发电位记录在功能磁共振成像引导下经颅磁刺激

Transcranial Magnetic Stimulation (TMS) is an effective method for establishing a causal link between a cortical area and cognitive/neurophysiological ...

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

在实验视神经脱髓鞘模型大鼠视觉诱发电位记录

The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to ...

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats

电图同时记录和麻醉大鼠视觉诱发电位

The electroretinogram (ERG) and visual evoked potential (VEP) are commonly used to assess the integrity of the visual pathway. The ERG measures the ...

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

可视化指南排序的电生理记录使用“SpikeSorter”

Few stand-alone software applications are available for sorting spikes from recordings made with multi-electrode arrays. Ideally, an application should be ...

Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings

用单单元记录法研究猕猴背视流中的对象表示

Previous studies have shown that neurons in parieto-frontal areas of the macaque brain can be highly selective for real-world objects, disparity-defined ...

Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice

c57bl6 小鼠耳蜗植入术与电唤起脑干反应记录

Cochlear implants (CIs) are neuroprosthetic devices that can provide a sense of hearing to deaf people. However, a CI cannot restore all aspects of ...