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DOI :
10.3791/56932-v
May 6th, 2018
Chapters
0:04
Title
1:20
Isolation of Tumor-secreted Extracellular Vesicles (EVs)
4:00
Generation of an Orthotopic Xenograft Mouse Model of Osteosarcoma
6:44
Assessment of Lung Nodule Number
7:57
Histological Analysis of GFP-positive MSCs in Mouse Tibias
8:53
Results: Analysis of Mice Injected with EV-educated MSCs
10:31
Conclusion
直接注射癌症衍生细胞外囊 (EVs) 导致骨髓支持肿瘤进展的重新编程;然而, 哪些细胞调解这种影响是不清楚的。在这里, 我们描述了一步一步的协议来调查 ev 介导的肿瘤间充质干细胞 (MSC) 相互作用的在体内, 揭示了一个关键的作用, 电动汽车教育的 MSCs 在转移。
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