Name: a rat model of orthotopic liver transplantation using a novel magnetic anastomosis technique for suprahepatic vena cava reconstruction
Uploaded: 2018-03-19T12:30:00
Description: Watch this Scientific Journal Video about A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction at JoVE.com

Dette arbeidet ble støttet av tilskudd fra departementet for utdanning innovasjon Team Development Program av Kina (nr. IRT16R57), National Natural Science Foundation av Kina (nr. 81470896) og en forskning fondet for unge Talent rekruttering planlegger av Xi'an Jiaotong University (RW).

Protokollen ble gjennomført i henhold til veiledning og bruk av forsøksdyr og ble godkjent av komiteen på etikk av dyr eksperimenter av Xi'an Jiaotong University, Xi'an, Shaanxi-provinsen, Kina.
Merk: Ingen av fremgangsmåtene i denne protokollen ble utført under kirurgisk mikroskop.
1. preoperativ forberedelse
<ol><li>Design og form av magnetiske ringene (figur 1)<ol>
<li>Samle magnetiske anastomose enheten ved et par Neodym-jern...

<ol>
	<li>Aller, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+half+century+(1961-2011)+of+applying+microsurgery+to+experimental+liver+research.">A half century (1961-2011) of applying microsurgery to experimental liver research.</a> World journal of hepatology. 4, 199-208 (2012).</li><li>Aller, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+value+of+microsurgery+in+liver+research.">The value of microsurgery in liver research.</a> Liver international : official journal of the International Association for the Study of the Liver. 29, 1132-1140 (2009).</li><li>Lee, S., Charters, A. C., Chandler, J. G., Orloff, M. 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Technique using cuff for portal vein anastomosis and biliary drainage.</a> Transplantation. 28, 47-50 (1979).</li><li>Oldani, G., Lacotte, S., Morel, P., Mentha, G., Toso, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+liver+transplantation+in+rats.">Orthotopic liver transplantation in rats.</a> Journal of visualized experiments : JoVE. , (2012).</li><li>Nagai, K., Yagi, S., Uemoto, S., Tolba, R. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+procedures+for+a+rat+model+of+partial+orthotopic+liver+transplantation+with+hepatic+arterial+reconstruction.">Surgical procedures for a rat model of partial orthotopic liver transplantation with hepatic arterial reconstruction.</a> Journal of visualized experiments : JoVE. , e4376 (2013).</li><li>Liu, X., He, C., Huang, T., Gu, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+New+Technique+for+Reconstruction+of+Hepatic+Artery+during+Liver+Transplantation+in+Sprague-Dawley+Rat.">Development of a New Technique for Reconstruction of Hepatic Artery during Liver Transplantation in Sprague-Dawley Rat.</a> PloS one. 10, e0145662 (2015).</li><li>Jamshidi, R., Stephenson, J. T., Clay, J. G., Pichakron, K. O., Harrison, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnamosis:+magnetic+compression+anastomosis+with+comparison+to+suture+and+staple+techniques.">Magnamosis: magnetic compression anastomosis with comparison to suture and staple techniques.</a> Journal of pediatric surgery. 44, 222-228 (2009).</li><li>Pichakron, K. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnamosis+II:+Magnetic+compression+anastomosis+for+minimally+invasive+gastrojejunostomy+and+jejunojejunostomy.">Magnamosis II: Magnetic compression anastomosis for minimally invasive gastrojejunostomy and jejunojejunostomy.</a> Journal of the American College of Surgeons. 212, 42-49 (2011).</li><li>Gonzales, K. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnamosis+III:+delivery+of+a+magnetic+compression+anastomosis+device+using+minimally+invasive+endoscopic+techniques.">Magnamosis III: delivery of a magnetic compression anastomosis device using minimally invasive endoscopic techniques.</a> Journal of pediatric surgery. 47, 1291-1295 (2012).</li><li>Wall, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MAGNAMOSIS+IV:+magnetic+compression+anastomosis+for+minimally+invasive+colorectal+surgery.">MAGNAMOSIS IV: magnetic compression anastomosis for minimally invasive colorectal surgery.</a> Endoscopy. 45, 643-648 (2013).</li><li>Xue, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Choledochojejunostomy+with+an+innovative+magnetic+compressive+anastomosis:+How+to+determine+optimal+pressure?.">Choledochojejunostomy with an innovative magnetic compressive anastomosis: How to determine optimal pressure?.</a> World journal of gastroenterology. 22, 2326-2335 (2016).</li><li>Yan, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Portacaval+shunt+established+in+six+dogs+using+magnetic+compression+technique.">Portacaval shunt established in six dogs using magnetic compression technique.</a> PloS one. 8, e76873 (2013).</li><li>Dorman, R. 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Mange klinisk utvikler i leveren transplantasjon kan tilskrives dyrestudier. Rotte OLT er en mye brukt og godt akseptert modell med forskning rundt orgel bevaring, transplantasjon immunologi, fysiologi og patologi. Men det er også svært komplisert og krever avansert Mikrokirurgiske ferdigheter. Til tross for mange forbedringer i prosedyren av rotte OLT forble SHVC anastomose en utfordrende trinn. I denne artikkelen beskriver vi en roman magnetiske anastomose teknikk for SHVC rekonstruksjon i rotte OLT. Bruk av denne te...

En rotte modell av orthotopic leveren transplantasjon (OLT) er avgjørende for transplantasjon forskning1,2. Første rotta OLT ble beskrevet av Lee et al. i 19733. I denne modellen, ble alle rekonstruert av hånd-Sutur teknikken. Hånd-Sutur teknikken krever avansert Mikrokirurgiske ferdigheter, som grenser betydelig dens utnyttelse. Siden da har ulike endringer av originale protokoll av rotte OLT rapportert. Blant dem, mansjetten teknikken for anastomose portalen blodåre (PV) og infrahepatic vena cava (IHVC) rapportert av Kamadan et al. i 1979 er ansett som en stor forbedring på denne modellen, som det betydelig forenklet rekonstruksjon prosedyrene4 . Men fordi kort fremre veggen mottakernes suprahepatic vena cava (SHVC) er mansjetten teknikken veldig vanskelig å bruke for gjenoppbyggingen av SHVC. De fleste forskere i dette feltet fremdeles bruke hånd-Sutur teknikken SHVC gjenoppbygging, noe som gjør den flaskehals trinnet rotte OLT5,6,7.
Magnetisk anastomose teknikken (dvs., magnamosis) er en metode for å koble to skip eller andre stålrør strukturer med attraktive kraften mellom to magneter8,9,10,11. Magnetisk kraft gradvis komprimerer og remodels vevet inn i en sterk, sutureless anastomose12,13. Dette komprimering anastomose har vist seg for å være effektive i mennesker14,15. Vi har utviklet et par magnetiske ringer spesielt for anastomose av SHVC i rotter. Våre siste studie har vist at den magnetiske anastomose teknikken er overlegen hånd-Sutur teknikken SHVC gjenoppbygging i rotte OLT16. Formålet med denne artikkelen er å gi en detaljert, trinnvis protokoll for SHVC rekonstruksjon i rotter ved hjelp av romanen magnetiske anastomose teknikk.

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			<td>It is mainly used for the angiography and photography of various operations</td>
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			<td>anesthetic:for the induction and maintenanceof anesthesia</td>
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			<td>&#160;intravascular contrast media&#160;&#160;</td>
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			<td height="23" style="height:23px;width:299px;">heparin sodium injection</td>
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			<td>&#160;prevent the formation of thrombosis&#160;</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td>surgical tool</td>
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			<td height="21" style="height:21px;width:299px;">hemostatic forceps(curved)</td>
			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td height="21" style="height:21px;width:299px;">Satinsky clamp</td>
			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td>surgical tool</td>
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			<td height="21" style="height:21px;width:299px;">microneedle holder</td>
			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
			<td style="width:119px;">MR-S121T</td>
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			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
			<td style="width:119px;">XEC240</td>
			<td>surgical tool</td>
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			<td height="21" style="height:21px;width:299px;">straight microforceps(titanium alloy)</td>
			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
			<td style="width:119px;">WCC010</td>
			<td>surgical tool</td>
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			<td height="21" style="height:21px;width:299px;">curved microforceps&#160; (titanium alloy)</td>
			<td style="width:377px;">Shanghai Medical Instruments (Group) Ltd., Corp.</td>
			<td style="width:119px;">WCC020</td>
			<td>surgical tool</td>
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a rat model of orthotopic liver transplantation using a novel magnetic anastomosis technique for suprahepatic vena cava reconstruction

En rotte modell av orthotopic leveren transplantasjon (OLT) er avgjørende for transplantasjon forskning. Det er en svært sofistikert animalsk modell og krever en bratt læringskurve. Innføring av mansjetten teknikken for anastomose portalen blodåre (PV) og infrahepatic vena cava (IHVC) har betydelig forenklet transplantasjon prosedyren i rotter. Men fordi kort fremre veggen mottakernes suprahepatic vena cava (SHVC) er mansjetten teknikken veldig vanskelig å bruke for gjenoppbyggingen av SHVC. De fleste forskere i dette feltet fremdeles bruke hånd-Sutur teknikken SHVC gjenoppbygging, noe som gjør den flaskehals trinnet rotte orthotopic leveren transplantasjon. Magnetisk anastomose teknikken (dvs., magnamosis) er en metode for å koble sammen to skip med attraktive kraften mellom to magneter. Våre siste studie har vist at den magnetiske anastomose teknikken er overlegen hånd-Sutur teknikken SHVC gjenoppbygging i rotter. I denne artikkelen viser vi en trinnvis protokoll for SHVC rekonstruksjon i rotter ved hjelp av romanen magnetiske anastomose teknikk. I denne modellen, ble gjenoppbygging av PV og IHVC utført av standard mansjett teknikken, mens gjenoppbyggingen av galle duct (BD) ble utført av en stent teknikk. Hepatic re arterialization ble ikke utført. Magnetisk anastomose teknikken gjort SHVC rekonstruksjon mye lettere og vesentlig forkortet anphepatic fasen. Etter en rimelig læringskurve, kan even forskere uten avansert Mikrokirurgiske ferdigheter gi pålitelig og reproduserbar resultater ved hjelp av denne rotte modell av OLT.

Etter ca. 10 forsøk, ble magnetiske anastomose teknikken SHVC gjenoppbygging vellykket mastret av en forsker som hadde ingen tidligere Mikrokirurgiske opplæring. Gjenoppbyggingen av SHVC tok mindre enn 2 minutter. Anhepatic fasen for mottakeren rotter var ca 10 min. Ingen blodpropp, blødning eller angiostegnosis ble observert på området SHVC anastomotisk 1, 5 og 30 dager etter reperfusion. Dårligere vena cavography ble utført to uker etter transplantasjon. Som vist i <strong class=...

Watch this Scientific Journal Video about A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction at JoVE.com

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

Gjenoppbygging av suprahepatic vena cava (SHVC) er fortsatt et vanskelig skritt i rotte orthotopic leveren transplantasjon. I denne artikkelen viser vi en trinnvis protokoll for SHVC rekonstruksjon i rotter ved hjelp av en roman magnetiske anastomose teknikk.

En rotte modell av Orthotopic levertransplantasjon bruker en roman magnetiske anastomose teknikk Suprahepatic Vena Cava gjenoppbygging

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep ...

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