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DOI :
10.3791/56977-v
May 9th, 2018
Chapters
0:04
Title
0:44
Preparing Animals and Equipment
2:55
Dissecting the Retina
4:36
Flat-mounting the Retina
6:21
Slicing the Retina and Assembling the Imaging Ladder
8:39
Results: Visualizing Ca2-dynamics in Cone Photoreceptors
10:19
Conclusion
影像视网膜组织可以提供无法从传统生化方法收集的单细胞信息。该协议描述了从斑马鱼的视网膜切片的制备共焦成像。荧光基因编码的传感器或指示染料允许可视化许多生物学过程中的不同的视网膜细胞类型。
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