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DOI :
10.3791/56981-v
October 3rd, 2018
Chapters
0:04
Title
1:19
Construction of the ORF Library
2:38
Preparation of the Filtering Vector
3:40
Ligation and Transformation
6:47
pFILTER-ORF Library Validation and Collection
9:10
Phage Library Deep Sequencing Platform, Index PCR Followed by Qualitative and Quantitative Evaluation of the Sequencing Library
10:07
Results: Use of a Phagemid Vector to Construct, Characterize and Select a Domainome Library
11:23
Conclusion
所描述的协议允许构造、特征和选择 (针对选择的目标) 的 "domainome" 图书馆由任何 DNA 来源。这是通过一个结合不同技术的研究管道实现的: 噬菌体展示, 折叠记者和下一代测序, 以及一个用于数据分析的 web 工具。
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