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DOI :
10.3791/56990-v
•
9:05 min
December 25th, 2017
Chapters
0:05
Title
0:47
N. vitripennis Colony Rearing
1:40
Collection and Alignment of N. vitripennis Pre-blastoderm Stage Embryos
4:13
Embryo Microinjection
5:58
Transplanting Injected G0 N. vitripennis Embryos onto Pre-stung Host
7:15
Results: sgRNA and Cas9 Microinjection in N. vitripennis Transplanted Embryos
8:23
Conclusion
显微注射的金小蜂金小蜂胚胎是产生遗传基因组改变的必要方法。这里描述的是一个详细的程序, 显微注射和移植的金小蜂金小蜂胚胎, 这将大大促进未来的基因组操纵在这个有机体。
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