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DOI :
10.3791/57006-v
•
9:47 min
September 13th, 2018
Chapters
0:04
Title
1:01
Materials Setup
2:19
Measurement Electrode Placement
4:35
Basal Potential Difference (PD) Measurement
6:02
Nasal Membrane Transepithelial Potential Difference (NPD) Tracing Sequential Perfusions
7:42
Results: Representative CF and Healthy Subject Tracings
8:57
Conclusion
在这里, 我们提出了一个标准化的协议, 以测量鼻电位差 (NPD)。囊性纤维化跨膜电导调节剂 (CFTR) 和上皮性钠通道 (ENaC) 的功能是评估的变化, 在 superfusion 的解决方案, 改变离子通道活动后的鼻上皮的电压, 提供了一个结果测量。
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