Generation of Brain Tumor Neurospheres and Culturing Conditions
2:23
Chromatin Preparation
4:52
Immunoprecipitation (IP)
8:21
Results: Representative ChIP qPCR Data as Percent Input
9:05
Conclusion
Transcript
The overall goal of this procedure is to identify the enrichment of histone marks in a specific genomic location. This method can help answer key questions in the epigenetics field, such as how mutations in epigenetic regulators which alter histon
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Epigenetiske mekanismer er ofte ændret i gliom. Kromatin immunoprecipitation kunne bruges til at studere konsekvenserne af genetiske ændringer i gliom, der skyldes ændringer i Histon ændringer, som regulerer kromatin struktur og gen transskription. Denne protokol beskriver indfødte kromatin immunoprecipitation på murine hjerne tumor neurospheres.