Generation of Brain Tumor Neurospheres and Culturing Conditions
2:23
Chromatin Preparation
4:52
Immunoprecipitation (IP)
8:21
Results: Representative ChIP qPCR Data as Percent Input
9:05
Conclusion
Transcript
The overall goal of this procedure is to identify the enrichment of histone marks in a specific genomic location. This method can help answer key questions in the epigenetics field, such as how mutations in epigenetic regulators which alter histon
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Epigenetic mekanismer endres ofte i glioma. Chromatin immunoprecipitation kan brukes til å studere konsekvensene av genetiske endringer i glioma som skyldes endringer i histone modifikasjoner som regulerer chromatin struktur og gene transkripsjon. Denne protokollen beskriver innfødt chromatin immunoprecipitation på murine hjerne svulst neurospheres.