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DOI :
10.3791/57017-v
•
11:51 min
April 27th, 2018
Chapters
0:05
Title
1:46
Recombinant Protein Expression
4:05
Target Protein Purification via Immobilized Metal Ion Affinity Chromatography
5:20
Intact Protein Mass Analysis by High-performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-flight Mass Spectrometry
6:38
Fluorescence Lifetime Measurements and Decay-associated Spectra of GdFP
7:59
Results: Gold Fluorescent Protein Analysis by Mass Spectrometry and Fluorescence
10:13
Conclusion
合成生物学使蛋白质的工程具有空前的特性, 使用非规范氨基酸的共平移插入。在这里, 我们介绍了如何一个光谱红色转移变种的 GFP 型荧光与新的荧光光谱性质, 称为 "金" 荧光蛋白 (GdFP), 是产生于大肠杆菌通过选择性压力成立 (SPI)。
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