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DOI :
10.3791/57033-v
February 21st, 2018
Chapters
0:04
Title
0:58
Media Preparation
1:24
Production of Retrovirus
3:28
Tritation of Virus Containing a Puromycin Reporter
5:04
Transduction of 32D/G-CSF-R Cells
6:10
32D/G-CSF-R Cell Differentiation Assay
7:32
Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media
9:21
Conclusion
本文介绍了培养小鼠髓质前体32维/G-脑脊液 R 细胞系的详细规程, 并进行了病毒感染, 并进行了增殖和分化测定。该细胞系适合研究髓细胞发育, 以及兴趣基因在髓细胞生长和中性分化中的作用。
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