Tritation of Virus Containing a Puromycin Reporter
5:04
Transduction of 32D/G-CSF-R Cells
6:10
32D/G-CSF-R Cell Differentiation Assay
7:32
Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media
9:21
Conclusion
Transcript
The overall goal of this experiment is to genetically modify the murine myeloid 32D-G-CSF-R cells by over expressing or silencing the gene of interest and determining the effect of these changes on proliferation and neutrophilic differentiation of
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Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.