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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

DOI :

10.3791/55268-v

March 16th, 2017

March 16th, 2017

8,354 Views

1Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania

Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

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Keywords Human Pluripotent Stem Cells

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