Name: a time-lapse, label-free, quantitative phase imaging study of dormant and active human cancer cells
Uploaded: 2018-02-16T13:00:00
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作者感谢乳腺癌研究基金会和先进医学研究基金会的支持。

这里描述的所有方法都已被波士顿儿童医院机构生物安全委员会批准。
1. 细胞制剂
<ol><li>
解冻 KHOS 细胞
	<ol>
<li>预热培养基,即, Dulbecco 的改性鹰培养基辅以 10% (卷/卷) 胎小牛血清 (血清) 和 1% (卷/卷) 青霉素/链霉素。</li>
		<li>从液氮罐中取去低温小瓶, 将瓶子的底部浸入37摄氏度水浴中的温水中, 轻轻摇动低温瓶以加速解冻过程。保持低温小瓶的盖子不接触...

<ol>
	<li>Folkman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenesis+in+cancer,+vascular,+rheumatoid+and+other+disease.">Angiogenesis in cancer, vascular, rheumatoid and other disease.</a> Nature Medicine. 1 (1), 27-31 (1995).</li><li>Hanahan, D., Folkman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterns+and+emerging+mechanisms+of+the+angiogenic+switch+during+tumorigenesis.">Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis.</a> Cell. 86 (3), 353-364 (1996).</li><li>Harper, J., Moses, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+regulation+of+tumor+angiogenesis:+mechanisms+and+therapeutic+implications.">Molecular regulation of tumor angiogenesis: mechanisms and therapeutic implications.</a> EXS. (96), 223-268 (2006).</li><li>Naumov, G. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+human+tumor+dormancy:+an+angiogenic+switch+from+the+nonangiogenic+phenotype.">A model of human tumor dormancy: an angiogenic switch from the nonangiogenic phenotype.</a> Journal of the National Cancer Institute. 98 (5), 316-325 (2006).</li><li>Fang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Matrix+metalloproteinase-2+is+required+for+the+switch+to+the+angiogenic+phenotype+in+a+tumor+model.">Matrix metalloproteinase-2 is required for the switch to the angiogenic phenotype in a tumor model.</a> Proceedings of the National Academy of Sciences of the United States of America. 97 (8), 3884-3889 (2000).</li><li>Almog, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcriptional+switch+of+dormant+tumors+to+fast-growing+angiogenic+phenotype.">Transcriptional switch of dormant tumors to fast-growing angiogenic phenotype.</a> Cancer Research. 69 (3), 836-844 (2009).</li><li>Hu, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+expression+signature+for+angiogenic+and+nonangiogenic+non-small-cell+lung+cancer.">Gene expression signature for angiogenic and nonangiogenic non-small-cell lung cancer.</a> Oncogene. 24 (7), 1212-1219 (2005).</li><li>Harper, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Repression+of+vascular+endothelial+growth+factor+expression+by+the+zinc+finger+transcription+factor+ZNF24.">Repression of vascular endothelial growth factor expression by the zinc finger transcription factor ZNF24.</a> Cancer Research. 67 (18), 8736-8741 (2007).</li><li>Jia, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcriptional+repression+of+VEGF+by+ZNF24:+mechanistic+studies+and+vascular+consequences+in+vivo.">Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo.</a> Blood. 121 (4), 707-715 (2013).</li><li>Jia, D., Huang, L., Bischoff, J., Moses, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+endogenous+zinc+finger+transcription+factor,+ZNF24,+modulates+the+angiogenic+potential+of+human+microvascular+endothelial+cells.">The endogenous zinc finger transcription factor, ZNF24, modulates the angiogenic potential of human microvascular endothelial cells.</a> FASEB journal: official publication of the Federation of American Societies for Experimental Biology. 29 (4), 1371-1382 (2015).</li><li>Almog, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prolonged+dormancy+of+human+liposarcoma+is+associated+with+impaired+tumor+angiogenesis.">Prolonged dormancy of human liposarcoma is associated with impaired tumor angiogenesis.</a> FASEB journal: official publication of the Federation of American Societies for Experimental Biology. 20 (7), 947-949 (2006).</li><li>Almog, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Consensus+micro+RNAs+governing+the+switch+of+dormant+tumors+to+the+fast-growing+angiogenic+phenotype.">Consensus micro RNAs governing the switch of dormant tumors to the fast-growing angiogenic phenotype.</a> PloS One. 7 (8), e44001 (2012).</li><li>Satchi-Fainaro, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prospective+identification+of+glioblastoma+cells+generating+dormant+tumors.">Prospective identification of glioblastoma cells generating dormant tumors.</a> PloS One. 7 (9), e44395 (2012).</li><li>Almog, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcriptional+changes+induced+by+the+tumor+dormancy-associated+microRNA-190.">Transcriptional changes induced by the tumor dormancy-associated microRNA-190.</a> Transcription. 4 (4), 177-191 (2013).</li><li>Gao, D., Nolan, D. J., Mellick, A. S., Bambino, K., McDonnell, K., Mittal, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells+control+the+angiogenic+switch+in+mouse+lung+metastasis.">Endothelial progenitor cells control the angiogenic switch in mouse lung metastasis.</a> Science. 319 (5860), 195-198 (2008).</li><li>Folkman, J., Watson, K., Ingber, D., Hanahan, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+angiogenesis+during+the+transition+from+hyperplasia+to+neoplasia.">Induction of angiogenesis during the transition from hyperplasia to neoplasia.</a> Nature. 339 (6219), 58-61 (1989).</li><li>Popescu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Quantitative phase imaging of cells and tissues. , (2011).</li><li>Lee, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+Phase+Imaging+Techniques+for+the+Study+of+Cell+Pathophysiology:+From+Principles+to+Applications.">Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications.</a> Sensors. 13 (4), 4170-4191 (2013).</li><li>Mir, M., Bhaduri, B., Wang, R., Zhu, R., Popescu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+Phase+Imaging.">Quantitative Phase Imaging.</a> Progress in Optics. 57, 133-217 (2012).</li><li>Marrison, J., R&#228;ty, L., Marriott, P., O'Toole, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ptychography--a+label+free,+high-contrast+imaging+technique+for+live+cells+using+quantitative+phase+information.">Ptychography--a label free, high-contrast imaging technique for live cells using quantitative phase information.</a> Scientific Reports. 3, 2369 (2013).</li><li>Falck Miniotis, M., Mukwaya, A., Gj&#246;rloff Wingren, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Digital+holographic+microscopy+for+non-invasive+monitoring+of+cell+cycle+arrest+in+L929+cells.">Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.</a> PloS One. 9 (9), e106546 (2014).</li><li>Popescu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+cell+mass+and+growth+dynamics.">Optical imaging of cell mass and growth dynamics.</a> AJP: Cell Physiology. 295 (2), C538-C544 (2008).</li><li>Guo, P., Huang, J., Moses, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+dormant+and+active+human+cancer+cells+by+quantitative+phase+imaging.">Characterization of dormant and active human cancer cells by quantitative phase imaging.</a> Cytometry. Part A: The Journal of the International Society for Advancement of Cytometry. 91 (5), 424-432 (2017).</li><li>Mir, M., Bergamaschi, A., Katzenellenbogen, B. S., Popescu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Highly+sensitive+quantitative+imaging+for+monitoring+single+cancer+cell+growth+kinetics+and+drug+response.">Highly sensitive quantitative imaging for monitoring single cancer cell growth kinetics and drug response.</a> PloS One. 9 (2), e89000 (2014).</li><li>Mir, M., Tangella, K., Popescu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+testing+at+the+single+cell+level+using+quantitative+phase+and+amplitude+microscopy.">Blood testing at the single cell level using quantitative phase and amplitude microscopy.</a> Biomedical Optics Express. 2 (12), 3259-3266 (2011).</li><li>Park, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+of+red+blood+cell+mechanics+during+morphological+changes.">Measurement of red blood cell mechanics during morphological changes.</a> Proceedings of the National Academy of Sciences of the United States of America. 107 (15), 6731-6736 (2010).</li><li>Pham, H. V., Bhaduri, B., Tangella, K., Best-Popescu, C., Popescu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real+time+blood+testing+using+quantitative+phase+imaging.">Real time blood testing using quantitative phase imaging.</a> PloS One. 8 (2), e55676 (2013).</li><li>Sridharan, S., Macias, V., Tangella, K., Kajdacsy-Balla, A., Popescu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prediction+of+prostate+cancer+recurrence+using+quantitative+phase+imaging.">Prediction of prostate cancer recurrence using quantitative phase imaging.</a> Scientific Reports. 5, 9976 (2015).</li><li>Park, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+cell+surface+area+and+deformability+of+individual+human+red+blood+cells+over+blood+storage+using+quantitative+phase+imaging.">Measuring cell surface area and deformability of individual human red blood cells over blood storage using quantitative phase imaging.</a> Scientific Reports. 6, 34257 (2016).</li><li>Bishitz, Y., Gabai, H., Girshovitz, P., Shaked, N. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical-mechanical+signatures+of+cancer+cells+based+on+fluctuation+profiles+measured+by+interferometry.">Optical-mechanical signatures of cancer cells based on fluctuation profiles measured by interferometry.</a> Journal of Biophotonics. 7 (8), 624-630 (2014).</li></ol>

在本研究中, 我们描述了一个体外, 无创, 无标签的方法, 使用 QPI 定量表征人骨肉瘤细胞的血管生成和非血管生成表型。采用综合、高通量的方法对多细胞参数进行了分析, 包括细胞面积、细胞厚度、细胞体积、增殖率、加倍时间、迁移直接、运动速度、迁移和运动。
与常规的细胞形态学和细胞行为评估方法相比, 该法不仅节省了时间和人工, 而且提供了更准确、更详细?...

在固体肿瘤的发展和进展中, 最早的检查点之一是获得血管生成表型, 这是癌症的标志。这一进展涉及各种生物化学和分子过程1,2,3。研究肿瘤进展这一关键步骤的技术挑战是缺乏连续和定量地对活癌细胞的血管生成和非血管生成性表型进行无偏定性和鉴别的工具。传统的检测方法用于研究血管生成和非血管生成细胞的细胞行为, 通常需要昂贵的试剂和仪器, 例如细胞增殖/迁移分析4,5, 6,7,8,9,10,11,12,13,14或互补的在体内评估4,5,6,8,15,16, 以及需要大量的专业知识和密集的时间和劳动消费。
最近, 定量相位成像 (QPI) 已经成为一种新的技术, 它能够对各种细胞形态学和行为参数进行时间推移和无标记的评估17,18,19,20,21,22. 与常规光学显微术不同, QPI 在光通过光学物体后按像素量化相移像素的变化, 并重建具有转换的光学厚度和体积的全息图像, 从而使直接对活细胞的分析和以下特点: (1) 定量成像, (2) 无创和时间推移成像, (3) 无标签成像, (4) 同时多参数成像。这些特性使 QPI 成为评估和理解细胞级病理过程的有力工具。
在最近的一项研究中, 我们利用 QPI 定量地定性和区分人骨肉瘤细胞的血管生成 KHOS 和非血管生成 KHOS-N 表型, 结合细胞形态学分析,扩散和运动23。利用图像分析软件, 对血管生成体与非血管生成性人骨肉瘤细胞的细胞形态和行为参数进行了定量比较, 并在这两个方面确定了五种特征差异。型.这种新的方法提供了一个综合和定量的平台, 以评估各种生物相关的细胞特征。

<table border="0" cellpadding="0" cellspacing="0" width="908">
	<tbody>
		<tr height="33">
			<td height="33" style="height:34px;width:227px;">T75 flask</td>
			<td style="width:227px;">Corning, NY, USA</td>
			<td style="width:227px;">353136</td>
			<td style="width:227px;"></td>
		</tr>
		<tr height="33">
			<td height="33" style="height:34px;width:227px;">6-well plates&#160;</td>
			<td style="width:227px;">Corning, NY, USA</td>
			<td style="width:227px;">3506</td>
			<td></td>
		</tr>
		<tr height="48">
			<td height="48" style="height:48px;width:227px;">Dulbecco&#8217;s modified Eagle medium (DMEM)</td>
			<td style="width:227px;">Thermo Fisher Scientific, MA, USA</td>
			<td style="width:227px;">11965092</td>
			<td></td>
		</tr>
		<tr height="33">
			<td height="33" style="height:34px;width:227px;">Fetal bovine serum (FBS)&#160;</td>
			<td style="width:227px;">Atlanta Biologicals, GA, USA</td>
			<td style="width:227px;">S11550</td>
			<td></td>
		</tr>
		<tr height="33">
			<td height="33" style="height:34px;width:227px;">Penicillin Streptomycin</td>
			<td style="width:227px;">Thermo Fisher Scientific, MA, USA</td>
			<td style="width:227px;">15140122</td>
			<td></td>
		</tr>
		<tr height="33">
			<td height="33" style="height:34px;width:227px;">Phosphate buffered saline (PBS)</td>
			<td style="width:227px;">Thermo Fisher Scientific, MA, USA</td>
			<td style="width:227px;">10010023</td>
			<td></td>
		</tr>
		<tr height="33">
			<td height="33" style="height:34px;width:227px;">Beckman Z1 Coulter counter</td>
			<td style="width:227px;">Beckman Coulter, IN, USA</td>
			<td style="width:227px;">Z1&#160;</td>
			<td></td>
		</tr>
		<tr height="51">
			<td height="51" style="height:51px;width:227px;">HoloMonitor M4</td>
			<td style="width:227px;">Phase Holographic Imaging Phi AB, Lund, Sweden</td>
			<td style="width:227px;">M4</td>
			<td>Microscope</td>
		</tr>
		<tr height="51">
			<td height="51" style="height:51px;width:227px;">Hololid</td>
			<td style="width:227px;">Phase Holographic Imaging Phi AB, Lund, Sweden</td>
			<td style="width:227px;">PHI 8020</td>
			<td></td>
		</tr>
		<tr height="51">
			<td height="51" style="height:51px;width:227px;">HStudioM4</td>
			<td style="width:227px;">Phase Holographic Imaging Phi AB, Lund, Sweden</td>
			<td style="width:227px;">HStudioM4</td>
			<td>Software</td>
		</tr>
	</tbody>
</table>

a time-lapse, label-free, quantitative phase imaging study of dormant and active human cancer cells

血管生成表型的获得是肿瘤休眠逃逸的重要组成部分。虽然一些经典的体外检测 (例如、增殖、迁移和其他) 和在体内模型已经开发, 以调查和表征血管生成和非血管生成细胞表型, 这些方法是时间和劳动密集型, 往往需要昂贵的试剂和仪器, 以及重要的专业知识。在最近的一项研究中, 我们用一种新的定量相成像 (QPI) 技术来进行血管生成和非血管生成性人骨肉瘤 KHOS 细胞的时间推移和无标记的特征。用 QPI 定量测量和分析了细胞参数的一个小组, 包括胞形态学、增殖和运动。这一新颖和定量的方法提供了机会, 以持续和非侵入性研究相关的细胞过程, 行为, 和肿瘤细胞的特点和其他细胞类型的简单和综合的方式。本报告描述了我们的实验性协议, 包括细胞制备、QPI 采集和数据分析。

图 1描述了典型的细胞形态学特征。图像显示为全息图(图1A-b) 和2D 图像 (图1C D)。光学细胞厚度 (从折射率和光学路径长度计算) 通过线剖面或整个细胞测量来量化。绘制了用于整个单元格的 KHOS 和 KHOS 单元的面积和厚度的散射图, 如图 1EG所示, 这...

Watch this Scientific Journal Video about A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells at JoVE.com

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

应用定量相成像技术对休眠和活性癌细胞表型进行了表征。用一种简单的方法对细胞增殖、迁移和形态学测定进行了综合分析。

休眠和活性人肿瘤细胞的时间推移、无标签、定量相成像研究

The acquisition of the angiogenic phenotype is an essential component of the escape from tumor dormancy. Although several classic in vitro assays (e.g., ...

Research

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Cancer Research

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies

转移性的型号同源小鼠肾细胞癌的临床前疗法的定量和纵向评估

Renal cell carcinoma (RCC) affects &#62; 60,000 people in the United States annually, and ~ 30% of RCC patients have multiple metastases at the time of ...

科研

癌症研究

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

磷酸浓缩结合无标签定量质谱法研究前列腺癌的 Phosphoproteome

Phosphoproteomics involves the large-scale study of phosphorylated proteins. Protein phosphorylation is a critical step in many signal transduction ...

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

利用微阵列研究微环境对癌症细胞表型的影响

Understanding the impact of the microenvironment on the phenotype of cells is a difficult problem due to the complex mixture of both soluble growth ...

Yeast As a Chassis for Developing Functional Assays to Study Human P53

酵母作为开发功能分析研究人类P53的机箱

The finding that the well-known mammalian P53 protein can act as a transcription factor (TF) in the yeast S. cerevisiae has allowed for the development of ...

Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

M1巨噬细胞-4T1小鼠乳腺癌细胞在共培养中噬菌体活性的时移2D成像

Tumor-associated macrophages (TAMs) have been identified as an important component for tumor growth, invasion, metastasis, and resistance to cancer ...

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

研究使用正交乳腺癌模型的三阴性乳腺癌

Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less ...

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因

Cancer stem cells (CSCs) are implicated in tumor initiation, development and recurrence after treatment, and have become the center of attention of many ...

Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

肿瘤细胞外侧尾静脉注射后肺转移的病理分析

Metastasis, the primary cause of morbidity and mortality for most cancer patients, can be challenging to model preclinically in mice. Few spontaneous ...

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

人类结肠癌细胞DNA损伤与修复福西的免疫荧光成像

The purpose of the manuscript is to provide a step-by-step protocol for performing immunofluorescence microscopy to study the radiation-induced DNA damage ...

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

一种用于乳腺肿瘤微环境活体内成像的无标记分割方法

The ability to visualize complex and dynamic physiological interactions between numerous cell types and the extracellular matrix (ECM) within a live tumor ...