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DOI :
10.3791/57036-v
•
9:52 min
April 26th, 2018
Chapters
0:05
Title
0:52
Preparation of Deionized Bovine Serum Albumin
1:48
Oocyte Collection
3:26
Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes
5:32
Fusion of Donor Cell and Enucleated Oocyte
7:42
Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C
8:26
Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment
9:15
Conclusion
我们用 trichostatin a、维生素 C 和去离子牛血清白蛋白来描述一种显著改进的小鼠克隆方法。我们展示了一个简化的、可重现的协议, 支持克隆胚胎的高效开发。因此, 该方法可以成为鼠标克隆的标准化程序。
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