Name: color spot test as a presumptive tool for the rapid detection of synthetic cathinones
Uploaded: 2018-02-05T14:45:00
Description: Watch this Scientific Journal Video about Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones at JoVE.com

作者希望通过澳大利亚政府的研究培训计划获得对摩根利的支持。

1. 颜色测试试剂溶液的制备
注: 将0.12 克硝酸铜重三水成干100毫升的烧杯。加入30毫升的去离子 (DI) 水, 在室温下小心地旋转, 以溶解所有固体。将这个溶液倒入100毫升的容积烧瓶中, 用 DI 水填充到标定的标记上。这个准备好的溶液是试剂1。 注: 试剂1可使用其他铜 (ii) 盐,如氯化铜 (ii) 制备。
<ol><li>称0.11 克 29-二甲基-110-邻菲咯啉 (试剂) 半水合物为干100毫升烧杯。加?...

<ol>
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这种颜色测试协议是根据苏拉亚·奥贝德et al发布的实验工作改编的。18中, 作者演示了在阿拉伯茶叶工厂提取的 cathinone 中出现颜色变化的情况。对已公布的议定书进行修改是必要的, 以预见其在推定的非法药物检测中的应用。最重要的考虑是减少反应的规模。本文件所述的议定书旨在适用于街头抽样和药物缉获。
所描述的协议提供了一个简单的假设?...

非法药物市场的运作与传统业务相似, 继续发展并适应不断变化的市场。现代技术的进步, 特别是在全球范围内, 强大的通信的激增, 通过暗网1和用户之间通过在线论坛2的广泛知识共享而增加了在线购买。与化学的进步相结合, 新的精神活性物质 (NPS) 的迅速出现, 给国际和国家药物管制带来了严重的挑战。
NPS 是有潜在危险的滥用药物, 对国际管制下的药物有类似的影响。最初作为 "法律" 的替代品销售, 在2009和 20163之间向联合国毒品和犯罪问题办事处 (禁毒办) 报告了 739 NPS。根据最近的年度报告, 在澳大利亚边境截获了一个记录数量的 NPS, 其中大多数是经过分析的, 进一步被确定为合成 cathinones4。在全球范围内, 自2010年首次报告以来, 合成 cathinones 的缉获量稳步增加, 是最常见的被缉获的 NPS5之一。
NPS 所构成的挑战主要是已发布的讨论主题6,7。法医实验室和执法人员处于劣势, 没有适当的方法在迅速出现时发现和识别核动力源。广泛的研究, 包括合成 cathinones 的检测, 在缴获的材料, 已使用气相色谱-质谱 (gc-ms)8和液相色谱-高分辨率质谱 (LC-人力资源)9验证性分析。对最小样品制备的需求不断增加, 已见红外和拉曼光谱10研究以及环境电离质谱分析, 如实时质谱直接分析 (DART-MS)11,12. 在该领域对快速、敏感的分析的需要也看到了将纸喷雾电离质谱 (PSI) 纳入到便携式设备中, 供执法人员13使用。许多仪器技术提供了验证性的分析与敏感的检测和定量的结果。但是, 对于高通量分析, 由于样品的准备、运行时间和仪器的培训和维护, 它们可能会耗费时间。
假定的颜色测试旨在建议在测试示例14中存在或缺少某些药物类。被缉获药物分析科学工作组 (SWGDRUG) 将颜色测试归类为最低的辨别力技术, 同时使用紫外光谱和免疫15。但是, 与其他技术相比, 执法部门和其他安全人员仍然广泛雇用他们, 以大大降低成本提供迅速的结果。彩色斑点测试方法所提供的主要优势是能够使用便携式测试套件在现场执行。
颜色测试的选择性取决于测试试剂与药物类之间发生的个别化学反应, 从而产生颜色变化。目前的推定测试协议缺乏检测合成 cathinones 的特殊测试;通常使用的试剂缺乏特异性和含有有害物质。其他推荐试剂尚未在大量可能的合成 cathinone 物质上进行筛选16。
这项工作的目的是提出一个简单的颜色测试协议, 可以很容易地使用的有关各方的初步筛选的合成 cathinones 在未知成分的非法物质。有关各方将包括执法部门、边防保护机构、法医实验室和其他有关安全人员。所提出的方法采用电子接受铜络合物试剂和富电子合成 cathinone 药物分子之间的还原氧化反应。使用这些化学方法开发, 你可以应用它们的形式推定颜色测试, 建议存在的合成 cathinones。

color spot test as a presumptive tool for the rapid detection of synthetic cathinones

合成 cathinones 是一大类新的精神活性物质 (NPS), 越来越普遍的药物缉获由执法和其他边境保护机构在全球范围内。颜色测试是一种推定的识别技术, 它使用快速而不复杂的化学方法来表示某一特定药物类别的存在或缺失。由于其相对较近的出现, 目前尚不具备对合成 cathinones 的具体鉴定的颜色测试。在这项研究中, 我们引入了一个协议的推定识别的合成 cathinones, 采用三水试剂溶液: 铜 (II) 硝酸盐, 29-二甲基-110-菲咯啉 (试剂) 和醋酸钠。将疑似药物的小针头大小 (大约 0.1-0.2 毫克) 添加到瓷斑板的井中, 然后在加热板上依次添加滴。在10分钟后, 从非常浅的蓝色到黄橙色的颜色变化表明可能存在的合成 cathinones。在法医实验室中, 这种高度稳定和特异的试验试剂有可能用于推定筛选未知样品, 用于合成 cathinones。然而, 对颜色变化结果的额外加热步骤的滋扰限制了测试的实验室应用, 并降低了容易翻译到现场测试的可能性。

通过几项研究验证了测试协议, 其结果在利et al.中进行了描述17. 颜色测试方法可以通过从浅蓝色到橙色 (图 1) 的颜色变化, 推定检测未知样品中的合成 cathinones。在加热周期后发生的黄色和橙色变化被认为是阳性测试结果和任何其他颜色变化, 包括非常微弱的黄色或在 heatingare 被视为负数之前发生的变化 (表 1)。</p...

Watch this Scientific Journal Video about Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones at JoVE.com

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

色斑试验作为合成 Cathinones 快速检测的推定工具

在这里, 我们提出了一个简单的, 廉价的, 选择性化学斑点测试协议, 以检测合成 cathinones, 一类新的精神活性物质。该议定书适用于遇到非法材料的各个执法领域。

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

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