Name: systematic assessment of well-being in mice for procedures using general anesthesia
Uploaded: 2018-03-20T13:00:00
Description: Watch this Scientific Journal Video about Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia at JoVE.com

多亏了 Rassam 的样本收集, 伊迪丝 Klobetz--对 FCM 的分析, PD 博士. 兽医。habil。萝丝维塔梅尔协助统计分析, 并 Wiebke Gentner 校对手稿。这项研究是柏林-勃兰登堡研究平台 BB3R (www.bb3r.de) 的一部分, 由德国联邦教育部和研究部资助 (赠款号: 031A262A) (www.bmbf.de/en/index.html)。

这项研究是根据《德国动物福利法》规定的准则进行的, 并得到了柏林国家当局 ("Landesamt 并且一厢情愿和 Soziales") 的批准, 允许号码: G0053/15)。
注: 本议定书的主要目的是研究反复麻醉对糖皮质激素代谢物的影响。为了确定要使用的动物数量, 进行了一个样本大小计算: n ≥ 2 x (s/µ1-µ2)2 x (zα + zβ)2。µ1-µ2是指在?...

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该议定书最初是为了评估接受单一麻醉或重复异氟醚麻醉的 C57BL/6JRj 小鼠的福祉而制定的。研究结果证实, 对奢侈品行为的测试以及其他措施 (例如自由探索范式、mg、穴居食物摄取) 都是评估幸福感的敏感方法。反复异氟醚麻醉对特质焦虑相关行为、特征和穴居行为产生短期影响。此外, 反复异氟醚麻醉似乎影响食物摄取量10。
自由探索范式表明, 减少...

欧盟指令 2010/631规定, 每当需要动物试验时, 由罗素和2开发的3R 原理 (替换、还原、细化) 将被应用.欧盟指令的最终目标是逐步淘汰所有动物试验, 但该指令承认, 目前还需要一些动物实验来进行研究, 以保护人类和动物的健康。因此, 如果动物实验不能被任何替代方法所取代, 只有最小数量的实验动物被用来获得可靠的结果。此外, 使用适当的细化措施, 应尽量减少伴随实验程序的疼痛、痛苦和苦恼的数量。欧盟指令2010/63 规定, 过程的严重性必须前瞻性归类为非恢复、温和、适度或严重的1。由于严重性分类是根据个案来决定的, 因此有科学的声音工具来估计给定过程的严重性是很重要的。
根据莫顿和格里菲斯的建议, 评分表3是检测任何偏离正常状态的重要工具, 包括对福祉4的负面影响。评分表用于回顾性地确定由实验引起的疼痛、痛苦和苦恼, 并着重于个体动物 (例如、体重、皮毛、步态) 的物理状态的可见变化。虽然, 欧盟指令2010/63 的附件八提供了每个严重性类别的例子, 但研究人员仍然缺乏利用科学基础的数据来估计给定程序严重性程度的工具。
缺乏显示消极福祉的指标并不是确定动物状况的唯一方法;指向正福利的指示器的存在也很重要5、6、7、8。例如, 动物只在满足所有基本需求时, 才会表现出诸如挖洞和筑巢等奢侈行为。如果减少了福利, 奢侈品行为首先会下降5,7。用于评估福祉的议定书应包括指示动物生理、生理/生物化学和心理状态的指标, 以便以详细和全面的方式对其福祉进行评估9。
在改进的范围内, 制定了一项议定书, 以满足这些要求, 并评估一项涉及全麻对小鼠福祉的程序的影响10。同时, 目标是尽量减少任何额外的压力, 使协议易于集成到给定的实验中。该协议考虑穴居行为, 家庭笼行为, 如活动, 食物摄取, 和巢, 和特质焦虑相关的行为。此外, 还包括小鼠的鬼脸量表和粪便中皮质酮代谢物的无创性分析。该议定书的目的是促进以科学和动物为中心的方式评估福祉, 并提供支持严重程度分类的福祉信息。除了评分表之外, 它还可以为过程的严重性分类提供有用的信息。由于该协议易于执行且不需要广泛的设备, 因此可以将其集成到一个正在进行的实验中, 而不影响研究结果。应该指出的是, 动物研究: 报告的活体实验 (到达) 指南11将在所有涉及动物实验的研究中观察到, 目的是改进设计、分析和报告。

<table border="0" cellpadding="0" cellspacing="0" width="603">
	<tbody>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Isofluran</td>
			<td style="width:101px;">CP-Pharma Handelsgesellschaft mbH</td>
			<td style="width:119px;">1214</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">InfraMot - Sensore Units</td>
			<td style="width:101px;">TSE Systems</td>
			<td>302015-SENS</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">InfraMot - Control Units</td>
			<td style="width:101px;">TSE Systems</td>
			<td style="width:119px;">302015-C/16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">InfraMot - Software</td>
			<td style="width:101px;">TSE Systems</td>
			<td style="width:119px;">302015-S</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Nestlet N</td>
			<td style="width:101px;">Ancare - Plexx</td>
			<td style="width:119px;">NES3600</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Camera EOS 350D</td>
			<td style="width:101px;">Canon</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

systematic assessment of well-being in mice for procedures using general anesthesia

根据罗素和科里开发的3R 原理 (替换、还原、细化), 科学研究应尽可能使用动物实验的替代品。如果不能替代动物实验, 那么所使用的实验动物总数应该是获取有价值数据所需的最低数量。此外, 应采用适当的细化措施, 以尽量减少伴随实验程序的痛苦、痛苦和痛苦。用于分类疼痛、痛苦和痛苦程度的类别是不恢复的、轻度的、中等的或严重的 (欧盟指令 2010/63)。要确定哪些类别适用于个别案例, 使用科学的声音工具是至关重要的。
这里提出的幸福评估协议是为使用全麻的程序设计的。该协议的重点是家庭笼活动, 鼠标鬼脸规模, 和奢侈行为, 如穴居和巢建筑行为作为福祉的指标。它也使用自由探索范式的特质焦虑相关行为。粪便皮质酮代谢物作为急性应激指标的测量在24小时后麻醉期间。
该议定书提供了有关全麻后小鼠的福祉的科学可靠的信息。由于其简单性, 该协议可以很容易地被修改和集成在一个计划的研究。虽然它没有提供一个规模, 根据欧盟指令2010/63 分类的苦恼, 它可以帮助研究人员估计一个程序的严重性程度, 使用科学的声音数据。它提供了一种以科学、动物为中心的方式来改进对幸福的评估的方法。

本议定书最初是为了评估 C57BL/6JRj 小鼠在单异氟醚麻醉 (一次45分钟麻醉疗程, n = 13 女性) 或重复异氟醚麻醉 (六45分钟麻醉疗程 3-4 天) 后的福祉而制定的。在麻醉疗程之间, n = 13 女性) 与控制小鼠 (n = 6 位女性) 的福利相比,10, 没有接受麻醉, 但根据相同的措施测试。我们评估了单一经验的异氟醚麻醉和重复异氟醚麻醉对 C57BL/6JRj 小鼠的幸福与未经治疗的控?...

Watch this Scientific Journal Video about Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia at JoVE.com

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

我们制定了一个协议, 以评估小鼠的福祉在程序中使用全麻。分析了一系列的行为参数, 说明了幸福水平和糖皮质激素代谢产物。该议定书可作为一般援助, 以科学的、以动物为中心的方式估计严重程度。

全身麻醉下小鼠幸福度的系统评价

In keeping with the 3R Principle (Replacement, Reduction, Refinement) developed by Russel and Burch, scientific research should use alternatives to animal ...

Research

JoVE Journal

Behavior

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

背景和线索恐惧制约试验小鼠中使用视频分析系统

The contextual and cued fear conditioning test is one of the behavioral tests that assesses the ability of mice to learn and remember an association ...

科研

行为学

Assessment of Age-related Changes in Cognitive Functions Using EmoCogMeter, a Novel Tablet-computer Based Approach

与年龄相关的变化认知功能使用EmoCogMeter，新型平板计算机基础法评估

The main goal of this study was to assess the usability of a tablet-computer-based application (EmoCogMeter) in investigating the effects of age on ...

Operant Procedures for Assessing Behavioral Flexibility in Rats

操作性程序，以评估大鼠的行为灵活性

Executive functions consist of multiple high-level cognitive processes that drive rule generation and behavioral selection. An emergent property of these ...

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach

使用自动图像处理方法鼠标社交性的实验评估

Mouse is the preferred model organism for testing drugs designed to increase sociability. We present a method to quantify mouse sociability in which the ...

Assessment of Cocaine-induced Behavioral Sensitization and Conditioned Place Preference in Mice

可卡因诱导行为敏和小鼠位置偏爱评估

It is thought that rewarding experiences with drugs create strong contextual associations and encourage repeated intake. In turn, repeated exposures to ...

Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

用于评估一般运动、肌肉力量和小鼠协调性的行为测试电池

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects and outcomes that a particular disease or treatment has on the ...

Tickling, a Technique for Inducing Positive Affect When Handling Rats

痒, 一种诱发大鼠正向影响的技术

Handling small animals such as rats can lead to several adverse effects. These include the fear of humans, resistance to handling, increased injury risk ...

Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice

小鼠低血压运动的下位强度及血管功能评估

Exercise training is an important strategy for maintaining health and preventing many chronic diseases. It is the first line of treatment recommended by ...

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

一种用于小鼠运动技能、情绪和认知重复评估的行为测试电池

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and ...

Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats

评估大鼠冲动不同表现的三个实验室程序

The present article provides a guide for the conduction and analysis of three conditioning-based protocols to evaluate impulsivity in rats. Impulsivity is ...